填充常规反相硅胶颗粒的无烧结色谱微芯片的制备。

Fabrication of fritless chromatographic microchips packed with conventional reversed-phase silica particles.

作者信息

Gaspar Attila, Piyasena Menake E, Gomez Frank A

机构信息

Department of Chemistry and Biochemistry, California State University, Los Angeles, 5151 State University Drive, Los Angeles, California 90032-8202, USA.

出版信息

Anal Chem. 2007 Oct 15;79(20):7906-9. doi: 10.1021/ac071106g. Epub 2007 Sep 11.

DOI:10.1021/ac071106g

PMID:17845005

Abstract

This paper describes the development and study of a disposable and inexpensive microfluidic chip, fabricated from poly(dimethylsiloxane) (PDMS) incorporating conventional chromatographic reversed-phase silica particles (C18) without the use of frits, permanent physical barriers, tapers, or restrictors. The packing of C18 modified silica particles into the microfluidic channels is made possible by the hydrophobic nature and excellent elasticity of PDMS. Keystone-, clamping-, and anchor-effects provide the stability and the compactness of the packing and attenuated wall-effects were observed.

摘要

本文描述了一种一次性且价格低廉的微流控芯片的开发与研究，该芯片由聚二甲基硅氧烷（PDMS）制成，其中包含传统的色谱反相硅胶颗粒（C18），且未使用烧结板、永久性物理屏障、锥度或限流装置。由于PDMS的疏水性和出色的弹性，使得将C18改性硅胶颗粒填充到微流控通道成为可能。关键效应、夹紧效应和锚固效应提供了填充物的稳定性和紧凑性，并且观察到壁效应减弱。