Zarfoss M K, Dubielzig R R
Department of Pathobiological Sciences, University of Wisconsin--Madison, 2015 Linden Dr., Madison, WI 53706, USA.
Vet Pathol. 2007 Sep;44(5):672-6. doi: 10.1354/vp.44-5-672.
An enucleated left eye from a 15-year-old female spayed Labrador Retriever was received by the Comparative Ocular Pathology Laboratory of Wisconsin (COPLOW) for histopathologic evaluation. Routine histologic preparation included staining with hematoxylin and eosin, and with alcian blue periodic acid-Schiff (PAS). At necropsy 9 months later, all grossly abnormal tissues (ipsilateral orbit and lung) were submitted to the COPLOW for histopathologic evaluation. Histopathologic evaluation of the globe revealed extensive invasion of the uvea and sclera by a pleomorphic cell population that formed disorganized cords and exhibited PAS-positive basement membrane material. Necropsy revealed a morphologically similar tumor in the ipsilateral orbit and lung. On immunohistochemical examination, the intraocular tumor stained diffusely immunopositive for vimentin, S-100, and neuron-specific enolase and multifocally, sparsely immunopositive for cytokeratin AE1/AE3. The orbital and thoracic tumors stained positively for vimentin but negatively for cytokeratin AE1/AE3. There are few reports of canine metastatic iridociliary adenocarcinoma in the literature; this is the first with immunohistochemical analysis.
一只15岁已绝育的雌性拉布拉多寻回犬的摘除左眼被送到威斯康星比较眼病理实验室（COPLOW）进行组织病理学评估。常规组织学制备包括苏木精和伊红染色以及阿尔辛蓝过碘酸希夫（PAS）染色。9个月后尸检时，所有肉眼可见异常的组织（同侧眼眶和肺）都被送到COPLOW进行组织病理学评估。眼球的组织病理学评估显示，葡萄膜和巩膜被一群多形性细胞广泛侵袭，这些细胞形成杂乱的条索，并呈现PAS阳性的基底膜物质。尸检显示同侧眼眶和肺有形态相似的肿瘤。免疫组织化学检查显示，眼内肿瘤波形蛋白、S-100和神经元特异性烯醇化酶弥漫性免疫阳性，细胞角蛋白AE1/AE3多灶性、散在免疫阳性。眼眶和胸部肿瘤波形蛋白染色阳性，但细胞角蛋白AE1/AE3染色阴性。文献中关于犬转移性虹膜睫状体腺癌的报道很少；这是首例进行免疫组织化学分析的病例。
