Naranjo Carolina, Dubielzig Richard R, Friedrichs Kristen R
Departament de Medicina i Cirurgia Animals, Facultat de Veterinària, Universitat Autònoma de Barcelona, Barcelona, Spain.
Vet Ophthalmol. 2007 May-Jun;10(3):179-85. doi: 10.1111/j.1463-5224.2007.00534.x.
To describe and characterize histiocytic sarcoma (HS) first detected in the eyes of dogs using the large database at the comparative ocular pathology laboratory of Wisconsin (COPLOW).
Cases diagnosed as HS were selected from the COPLOW database. Slides were reviewed to describe the cellular morphology, localize the tumor within the globe, record the tumor distribution and measure the size of the tumor. Further sections were taken to perform immunohistochemistry for Melan-A, CD18 and S-100, and for ferric iron staining. The following clinical information was recorded: breed, age, gender, laterality, clinical signs upon presentation and follow-up information obtained by response to a mailed survey and phone contact.
Twenty-six cases were confirmed as being HS according to the immunohistochemical results (CD18 positive and Melan-A negative). The most prevalent breed was Rottweiler (eight cases), followed by Retriever breeds (seven Golden Retrievers and five Labrador Retrievers). The mean age was 8.61 +/- 2.43 years. There were three intact male, eight castrated male, one intact female and 14 spayed female dogs. In 15 dogs there were no concurrent systemic clinical signs at the time of diagnosis. Sixteen of 19 dogs with follow-up information available died as a result of causes related to the tumor, although only three of them received a necropsy. Survival time varied between 5 days and 6 months after enucleation. Three of the dogs were alive at the time the information was gathered. Mean tumor surface was 0.613 +/- 0.38 cm(2). S-100 was diffusely positive in 10 cases, isolated positive cells were found in 11 cases and five cases were completely negative. Seven of the cases were positive for ferric iron.
Histiocytic sarcoma must be considered in the differential diagnosis of dogs with intraocular masses, especially in Rottweilers and Retriever breeds. Because it carries poor prognosis, it must be distinguished from melanoma. A good discriminator for this purpose in paraffin-embedded tissues is finding CD18-positive cells and no reactivity against Melan-A. S-100 and ferric iron staining does not seem to be useful. Ocular HS is considered to be a manifestation of a systemic disease even when the disease is first recognized in the eye.
利用威斯康星比较眼病理实验室（COPLOW）的大型数据库，描述和表征首次在犬眼发现的组织细胞肉瘤（HS）。
从COPLOW数据库中选取诊断为HS的病例。复查切片以描述细胞形态，确定肿瘤在眼球内的位置，记录肿瘤分布并测量肿瘤大小。进一步切片进行Melan-A、CD18和S-100免疫组化以及铁染色。记录以下临床信息：品种、年龄、性别、患侧、就诊时的临床症状以及通过邮寄调查问卷和电话联系获得的随访信息。
根据免疫组化结果（CD18阳性且Melan-A阴性）确诊26例为HS。最常见的品种是罗威纳犬（8例），其次是寻回犬品种（7只金毛寻回犬和5只拉布拉多寻回犬）。平均年龄为8.61±2.43岁。有3只未绝育雄性犬、8只去势雄性犬、1只未绝育雌性犬和14只绝育雌性犬。15只犬在诊断时无并发的全身临床症状。在有随访信息的19只犬中，16只因与肿瘤相关的原因死亡，尽管其中只有3只进行了尸检。眼球摘除术后存活时间在5天至6个月之间。在收集信息时，有3只犬存活。平均肿瘤表面积为0.613±0.38 cm²。10例S-100弥漫性阳性，11例发现孤立阳性细胞，5例完全阴性。7例铁染色阳性。
在鉴别诊断眼内肿物的犬时，必须考虑组织细胞肉瘤，尤其是在罗威纳犬和寻回犬品种中。由于其预后不良，必须与黑色素瘤相鉴别。在石蜡包埋组织中，为此目的的一个良好鉴别指标是发现CD18阳性细胞且对Melan-A无反应性。S-100和铁染色似乎无用。眼内HS即使最初在眼部被发现，也被认为是一种全身性疾病的表现。
