Kim Joonki, Jo Beom Ho, Lee Kyoung Lyong, Yoon Eui-Soo, Ryu Gi Hyung, Chung Ki Wha
Department of Biological Science, Kongju National University, Gongju 314-701, Korea.
Mol Cells. 2007 Aug 31;24(1):60-8.
Microsatellites, also called simple sequence repeats (SSR), are very useful molecular genetic markers commonly used in crop breeding, species identification and linkage analysis. In the present study, we constructed a microsatellite-enriched genomic library of Panax ginseng, and identified 251 novel microsatellite sequences. Tri-nt repeat units were the most abundant (46.6%), followed by di-nt repeats (35.5%). The (AG)n motif was most common (23.1%), followed by the (AAC)n motif (22.3%). From the genotyping of 94 microsatellites using marker-specific primer sets, we identified 11 intraspecific polymorphic markers as well as 14 possible interspecific polymorphic markers differing between P. ginseng and P. quinquefolius. The exact allele structures of the polymorphic markers were determined and the alleles were named. This study represents the first report of the bulk isolation of microsatellites by screening a microsatellite-enriched genomic library in P. ginseng. The microsatellite markers could be useful for linkage analysis, genetic breeding and authentication of Panax species.
微卫星，也称为简单序列重复（SSR），是非常有用的分子遗传标记，常用于作物育种、物种鉴定和连锁分析。在本研究中，我们构建了人参的微卫星富集基因组文库，并鉴定出251个新的微卫星序列。三核苷酸重复单元最为丰富（46.6%），其次是二核苷酸重复（35.5%）。（AG）n基序最为常见（23.1%），其次是（AAC）n基序（22.3%）。通过使用标记特异性引物对94个微卫星进行基因分型，我们鉴定出11个种内多态性标记以及14个可能的种间多态性标记，这些标记在人参和西洋参之间存在差异。确定了多态性标记的确切等位基因结构并对这些等位基因进行了命名。本研究是通过筛选人参微卫星富集基因组文库进行微卫星批量分离的首次报道。这些微卫星标记可用于人参属物种的连锁分析、遗传育种和鉴定。
