Xu Gaosi, He Qiang, Shou Zhangfei, Wang Huiping, Zhang Xiaohui, Wang Yaomin, Chen Ying, Chen Jianghua
Kidney Disease Centre, First Affiliated Hospital, College of Medicine, Zhejiang University, Hangzhou, China.
J Clin Lab Anal. 2007;21(5):298-302. doi: 10.1002/jcla.20189.
Several studies have identified FcgRIIIb (Fcgr3b) polymorphisms that determine susceptibility to autoimmune diseases such as systemic lupus erythematosus and rheumatoid arthritis. The objective of the study was to clarify whether Fcgr3b allele polymorphism influence susceptibility to immunoglobulin A nephropathy (IgAN), clinical features or severity in patients with IgAN. Deoxyribonucleic acid (DNA) fragments were amplified by polymerase chain reaction (PCR) using genomic DNA from 172 unrelated, healthy blood donors and 128 IgAN patients in our Kidney Disease Centre. The present findings showed that Fcgr3b genotype influenced the disease susceptibility and severity of IgAN, although Fcgr3b polymorphism did not affect the age of the disease onset. We found that the genotype frequency of Fcgr3b heterozygote NA1/NA2 in IgAN patients in Chinese significantly higher than that of healthy donors. Furthermore, higher genotype frequency of NA1/NA2 was found also in IgAN patients with glomerulosclerosis or crescent formation than those without it. NA1/NA2 heterozygote of Fcgr3b is a risk factor for progression of IgA nephropathy in Chinese.
多项研究已确定了FcγRIIIb（Fcgr3b）多态性，这些多态性决定了对自身免疫性疾病如系统性红斑狼疮和类风湿关节炎的易感性。本研究的目的是阐明Fcgr3b等位基因多态性是否影响免疫球蛋白A肾病（IgAN）的易感性、IgAN患者的临床特征或严重程度。使用来自我们肾脏病中心172名无亲缘关系的健康献血者和128名IgAN患者的基因组DNA，通过聚合酶链反应（PCR）扩增脱氧核糖核酸（DNA）片段。目前的研究结果表明，Fcgr3b基因型影响IgAN的疾病易感性和严重程度，尽管Fcgr3b多态性不影响疾病发病年龄。我们发现，中国IgAN患者中Fcgr3b杂合子NA1/NA2的基因型频率显著高于健康献血者。此外，与没有肾小球硬化或新月体形成的IgAN患者相比，有肾小球硬化或新月体形成的IgAN患者中NA1/NA2的基因型频率也更高。Fcgr3b的NA1/NA2杂合子是中国IgA肾病进展的危险因素。
