Rampersad Joanne, Ammons David
Department of Life Sciences, The University of the West Indies, St. Augustine, Trinidad.
J Am Mosq Control Assoc. 2007 Jun;23(2):149-52. doi: 10.2987/8756-971X(2007)23[149:VBBFLF]2.0.CO;2.
Research programs in mosquito-borne diseases have necessitated the bloodfeeding of mosquitoes in captivity. Parafilm has been shown to be an effective membrane through which mosquitoes can access blood, and numerous feeding devices using parafilm membranes have been reported. However, these devices can be incompatible with experimental conditions, which may require small blood volumes, exact and constant blood temperatures, and inexpensive or disposable materials. We report herein on 2 methodologies that can be used to make parafilm blood bags with a support side and a feeding side made up of a thinner sheet of parafilm. Blood bags were easily and cheaply made to handle volumes as little as 150 microl or as large as 5 ml, to be disposable, and to be easily fastened to a heat source to provide constant and reproducible blood temperatures. Blood bags represent perhaps the most versatile and inexpensive approach to parafilm membrane feeding yet reported.
蚊媒疾病的研究项目需要在圈养条件下让蚊子吸血。已证明石蜡膜是一种有效的膜，蚊子可通过它获取血液，并且已报道了许多使用石蜡膜的吸血装置。然而，这些装置可能与实验条件不兼容，实验条件可能需要少量血液、精确且恒定的血液温度以及廉价或一次性材料。我们在此报告两种方法，可用于制作具有支撑面和由较薄石蜡膜片组成的喂食面的石蜡膜血袋。血袋制作简便且成本低廉，可处理低至150微升或高达5毫升的血量，可一次性使用，并且易于固定在热源上以提供恒定且可重复的血液温度。血袋可能是迄今报道的石蜡膜喂食最通用且最便宜的方法。
