Aak A, Rukke B A
Department of Pest Control, Norwegian Institute of Public Health, Oslo, Norway.
Med Vet Entomol. 2014 Mar;28(1):50-9. doi: 10.1111/mve.12015. Epub 2013 May 22.
A blood-feeding system that utilizes a small amount of whole heparinized human blood in parafilm bags is described in detail, and similarities and differences between artificially fed and naturally rodent-fed bed bugs (Hemiptera: Cimicidae) are discussed. Blood with high levels of heparin (10%) was unsuitable for artificial colony rearing, whereas bed bugs fed on 1% heparinized blood and those that naturally ingested rat blood completed their lifecycle with similar stage structures over time, with no significant differences in mortality. No differences in feeding efficiency or fertility were found in a direct comparison of bed bugs maintained under each of these two treatments, but analysis of the full lifecycle revealed that artificially fed bed bugs became significantly smaller and laid fewer eggs than rodent-fed bed bugs. The level of membrane stretching regulated the number of bed bugs that fed. When the membrane was stretched to twice its length and width, 96% of bed bugs successfully fed through the parafilm. Whole heparinized blood that was stored at 6 °C for ≥ 14 days failed to retain its nutritional value and the amount of blood consumed and number of consecutive moults were significantly reduced.
详细描述了一种在石蜡膜袋中使用少量全肝素化人血的血液喂养系统，并讨论了人工喂养和自然以啮齿动物为食的臭虫（半翅目：臭虫科）之间的异同。高浓度肝素（10%）的血液不适合人工饲养种群，而以1%肝素化血液为食的臭虫和自然摄取大鼠血液的臭虫随着时间推移具有相似的阶段结构完成其生命周期，死亡率无显著差异。在这两种处理方式下饲养的臭虫直接比较中，未发现喂养效率或繁殖力有差异，但对整个生命周期的分析表明，人工喂养的臭虫比以啮齿动物为食的臭虫显著变小且产卵更少。膜拉伸程度调节了进食的臭虫数量。当膜被拉伸至其长度和宽度的两倍时，96%的臭虫通过石蜡膜成功进食。在6°C下储存≥14天的全肝素化血液未能保持其营养价值，消耗的血液量和连续蜕皮次数显著减少。
