Department of Entomology, College of Agriculture and Natural Resources, National Chung Hsing University, Taichung, Taiwan.
Department of Tropical Medicine and Parasitology, College of Medicine, National Taiwan University, Taipei, Taiwan.
PLoS One. 2020 May 29;15(5):e0233618. doi: 10.1371/journal.pone.0233618. eCollection 2020.
A simple device using folded Parafilm-M as an artificial blood feeder was designed for studying two important dengue vector mosquitoes, Aedes aegypti and Aedes albopictus. The efficiency of the artificial blood feeder was investigated by comparing the numbers of engorged mosquitoes that fed on the artificial blood feeder versus mice as a live blood source. Significantly more engorged females Aedes aegypti fed on the artificial blood feeder than on mice. In addition, the artificial feeder could serve as a useful apparatus for oral infection via artificial blood meals, and for saliva collection in mosquitoes. Our method enabled us to collect saliva from multiple mosquitoes at once, providing sufficient infected saliva for determination of the virus titer by plaque assay analysis. Our artificial feeder has the advantage that it is simple, inexpensive, and efficient.
我们设计了一种简单的装置，使用折叠的 Parafilm-M 作为人工血液饲养器，用于研究两种重要的登革热媒介蚊子，埃及伊蚊和白纹伊蚊。通过比较在人工血液饲养器上饱食的蚊子数量与作为活体血液源的老鼠数量，来评估人工血液饲养器的效率。在人工血液饲养器上饱食的埃及伊蚊雌蚊数量明显多于在老鼠上饱食的数量。此外，人工饲养器可作为通过人工血液餐进行口服感染以及收集蚊子唾液的有用仪器。我们的方法使我们能够一次收集多个蚊子的唾液，提供足够的感染唾液，用于通过噬菌斑分析确定病毒滴度。我们的人工饲养器具有简单、廉价和高效的优点。
