Hasić Sabaheta, Jadrić Radivoj, Kiseljaković Emina, Mornjaković Zakira, Winterhalter-Jadrić Mira
Institute for Physiology and Biochemistry, Faculty of Medicine, University of Sarajevo, Cekalusa 90, 71000 Sarajevo, Bosnia and Herzegovina.
Bosn J Basic Med Sci. 2007 Aug;7(3):212-7. doi: 10.17305/bjbms.2007.3046.
In our investigation,we used short-time model of myocardial infarction of rats induced by high dose of isoproterenol (ISP). We investigated cardiac troponin T blood level (cTnT) and histological characteristics of rat myocardium. ISP, single, intraperitoneal dose 250 mg/kg was given to male, adult, Wistar rats (n=12). Rats were distributed depending on their body weight in subgroups: ISP I (BW 260-280g) and ISP II (BW 250-400g).Control group (n=9) was treated with intraperitoneal dose of 0,95% NaCl. Cardiac TnT was measured by electrochemiluminiscence (ECLA) sandwich immunoassay in rat serum 4 hours after ISP application. Rats' hearts were dissected and examined by qualitative histological method (HE). Statistical significance was set at 0,05. There was significant difference in cTnT of ISP II (p=0,0001) vs. control and ISP I (p<0,05) vs. control. Significant difference was between ISP I and ISP II subgroups (p<0.001). The accent of histological changes of myocardium was on nuclei of cell. Cells showed acidophilic changes and nuclei disappearance as signs of coagulative necrosis development. Extensivity of histological changes were different between ISP I and ISP II subgroup. Used dose of ISP induced development of myocardial necrosis in rats. Subendocardial portion of myocardium was more vulnerability than subepicardial portion. Rats of ISP II had more extensive histological changes than these in ISP I. Administered doses of ISP enabled cTnT utilization as a marker of myocardial necrosis.
在我们的研究中，我们使用了高剂量异丙肾上腺素（ISP）诱导的大鼠心肌梗死短期模型。我们研究了大鼠心肌肌钙蛋白T的血液水平（cTnT）和心肌的组织学特征。将单次腹腔注射剂量为250mg/kg的ISP给予成年雄性Wistar大鼠（n = 12）。大鼠根据体重分为亚组：ISP I（体重260 - 280g）和ISP II（体重250 - 400g）。对照组（n = 9）腹腔注射0.95%氯化钠。在给予ISP 4小时后，通过电化学发光（ECLA）夹心免疫分析法测定大鼠血清中的心肌肌钙蛋白T。解剖大鼠心脏并采用定性组织学方法（苏木精-伊红染色，HE）进行检查。统计学显著性设定为0.05。ISP II组的cTnT与对照组相比有显著差异（p = 0.0001），ISP I组与对照组相比有显著差异（p < 0.05）。ISP I组和ISP II亚组之间存在显著差异（p < 0.001）。心肌组织学变化的重点在于细胞核。细胞出现嗜酸性变化和细胞核消失，这是凝固性坏死发展的迹象。ISP I组和ISP II亚组的组织学变化程度不同。所用剂量的ISP诱导了大鼠心肌坏死的发生。心肌内膜下层比心外膜下层更易受损。ISP II组大鼠的组织学变化比ISP I组更广泛。给予的ISP剂量使cTnT能够作为心肌坏死的标志物。
