Naito Mariko, Miyaki Koichi, Naito Toru, Zhang Ling, Hoshi Keika, Hara Asako, Masaki Katsunori, Tohyama Shugo, Muramatsu Masaaki, Hamajima Nobuyuki, Nakayama Takeo
Department of Preventive Medicine/Biostatistics, Medical Decision Making, Nagoya University Graduate School of Medicine, Tsurumai-cho, Showa-ku, Nagoya, Japan.
Int J Med Sci. 2007 Aug 22;4(4):216-22. doi: 10.7150/ijms.4.216.
The vitamin D receptor (VDR) is involved in a variety of biological processes, such as bone metabolism and modulation of the immune response. Recent findings suggest that the pathway involving bone mineral density-mediated effects is important for the development of periodontitis, but their effects of combined VDR gene polymorphisms have not been confirmed on periodontitis. We assessed the relationship between ApaI, BsmI, and FokI VDR polymorphisms and the risk of severe chronic periodontitis among Japanese adult men.
In a cross-sectional study, we examined 97 unrelated healthy Japanese men (mean age: 45.6 years, range: 22-59). A clinical examination was performed at a worksite health checkup, and information was obtained using a self-reported questionnaire. DNA was extracted from whole blood, and the VDR ApaI, BsmI, and FokI polymorphisms were genotyped using polymerase chain reaction.
F-carriers of FokI VDR polymorphisms were less likely to develop severe chronic periodontitis than non-F-carriers (p = 0.09). The ApaI and BsmI VDR polymorphisms did not show significant differences in the alleles or genotypes between the subjects with or without severe chronic periodontitis. The haplotype analysis of the three combined VDR polymorphisms revealed that the Abf homozygote had a notably higher prevalence of severe chronic periodontitis than the others, and adjustments for age, smoking status, number of teeth present, and prevalence of diabetes did not change this association (OR = 7.5; 95% CI = 1.6-34.4; p = 0.01).
The VDR haplotype constructed from the ApaI, BsmI, and FokI polymorphisms is related to the risk of severe chronic periodontitis in Japanese men.
维生素D受体（VDR）参与多种生物学过程，如骨代谢和免疫反应调节。最近的研究结果表明，涉及骨矿物质密度介导效应的途径对牙周炎的发展很重要，但VDR基因多态性的联合作用对牙周炎的影响尚未得到证实。我们评估了日本成年男性中ApaI、BsmI和FokI VDR基因多态性与重度慢性牙周炎风险之间的关系。
在一项横断面研究中，我们检查了97名无亲缘关系的健康日本男性（平均年龄：45.6岁，范围：22 - 59岁）。在工作场所健康检查时进行了临床检查，并通过自我报告问卷获取信息。从全血中提取DNA，并使用聚合酶链反应对VDR ApaI、BsmI和FokI基因多态性进行基因分型。
FokI VDR基因多态性的F携带者比非F携带者患重度慢性牙周炎的可能性更小（p = 0.09）。ApaI和BsmI VDR基因多态性在患有或未患有重度慢性牙周炎的受试者之间的等位基因或基因型上未显示出显著差异。对三种VDR基因多态性组合进行单倍型分析发现，Abf纯合子患重度慢性牙周炎的患病率明显高于其他单倍型，并且对年龄、吸烟状况、现存牙齿数量和糖尿病患病率进行调整后，这种关联并未改变（OR = 7.5；95% CI = 1.6 - 34.4；p = 0.01）。
由ApaI、BsmI和FokI基因多态性构建的VDR单倍型与日本男性重度慢性牙周炎风险相关。
