Leipuviene R, Theil E C
Center for BioIron, Children's Hospital Oakland Research Institute, Children's Hospital & Research Center at Oakland, 5700 Martin Luther King, Jr. Way, Oakland, California 94609, USA.
Cell Mol Life Sci. 2007 Nov;64(22):2945-55. doi: 10.1007/s00018-007-7198-4.
The life of aerobes is dependent on iron and oxygen for efficient bioenergetics. Due to potential risks associated with iron/oxygen chemistry, iron acquisition, concentration, storage, utilization, and efflux are tightly regulated in the cell. A central role in regulating iron/oxygen chemistry in animals is played by mRNA translation or turnover via the iron responsive element (IRE)/iron regulatory protein (IRP) system. The IRE family is composed of three-dimensional RNA structures located in 3' or 5' untranslated regions of mRNA. To date, there are 11 different IRE mRNAs in the family, regulated through translation initiation or mRNA stability. Iron or oxidant stimuli induce a set of graded responses related to mRNA-specific IRE substructures, indicated by differential responses to iron in vivo and binding IRPs in vitro. Molecular effects of phosphorylation, iron and oxygen remain to be added to the structural information of the IRE-RNA and IRP repressor in the regulatory complex.
需氧生物的生存依赖于铁和氧气以实现高效的生物能量学。由于与铁/氧化学反应相关的潜在风险，细胞中铁的获取、浓度、储存、利用和外流受到严格调控。在动物体内，通过铁反应元件（IRE）/铁调节蛋白（IRP）系统进行的mRNA翻译或周转在调节铁/氧化学反应中发挥着核心作用。IRE家族由位于mRNA 3'或5'非翻译区的三维RNA结构组成。迄今为止，该家族中有11种不同的IRE mRNA，通过翻译起始或mRNA稳定性进行调控。铁或氧化剂刺激会引发一系列与mRNA特异性IRE亚结构相关的分级反应，这在体内对铁的不同反应以及体外与IRP的结合中得到体现。磷酸化、铁和氧的分子效应仍有待补充到调节复合物中IRE-RNA和IRP阻遏物的结构信息中。
