基于纳米颗粒的细胞治疗监测。
Nanoparticle-based monitoring of cell therapy.
机构信息
Center for Regenerative Therapeutics and Department of Medicine, Brigham and Women's Hospital, Harvard Medical School, Cambridge, MA 02139, USA.
出版信息
Nanotechnology. 2011 Dec 9;22(49):494001. doi: 10.1088/0957-4484/22/49/494001. Epub 2011 Nov 21.
Abstract
Exogenous cell therapy aims to replace/repair diseased or dysfunctional cells and promises to revolutionize medicine by restoring tissue and organ function. To develop effective cell therapy, the location, distribution and long-term persistence of transplanted cells must be evaluated. Nanoparticle (NP) based imaging technologies have the potential to track transplanted cells non-invasively. Here we summarize the most recent advances in NP-based cell tracking with emphasis on (1) the design criteria for cell tracking NPs, (2) protocols for cell labeling, (3) a comparison of available imaging modalities and their corresponding contrast agents, (4) a summary of preclinical studies on NP-based cell tracking and finally (5) perspectives and future directions.
摘要
外源性细胞疗法旨在替代/修复患病或功能失调的细胞，并有望通过恢复组织和器官功能来彻底改变医学。为了开发有效的细胞疗法，必须评估移植细胞的位置、分布和长期持久性。基于纳米粒子（NP）的成像技术有可能对移植细胞进行非侵入性跟踪。在这里，我们总结了基于 NP 的细胞跟踪的最新进展，重点介绍了（1）用于细胞跟踪的 NP 的设计标准，（2）细胞标记的方案，（3）可用成像方式及其相应对比剂的比较，（4）基于 NP 的细胞跟踪的临床前研究的总结，最后（5）观点和未来方向。
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