Halász Júlia, Pedryc Andrzej, Hegedűs Attila
Department of Genetics and Plant Breeding, Corvinus University of Budapest, PO Box 53, Budapest, H-1518, Hungary.
Department of Applied Chemistry, Corvinus University of Budapest, PO Box 53, Budapest, H-1518, Hungary.
New Phytol. 2007;176(4):792-803. doi: 10.1111/j.1469-8137.2007.02220.x.
In China, its centre of origin, apricot (Prunus armeniaca) is self-incompatible. However, most European cultivars are self-compatible. In most cases, self-compatibility is a result of a loss-of-function mutation within the pollen gene (SFB) in the SC haplotype. Controlled pollinations performed in this work revealed that the cross 'Ceglédi óriás' (S8S9)x'Ceglédi arany' (SCS9) set well, as expected, but the reciprocal cross did not. Apricot S8, S9 and SC haplotypes were analysed using a multilevel approach including fruit set evaluation, pollen tube growth analysis, RNase activity assays, polymerase chain reaction (PCR) analysis and DNA sequencing of the S-RNase and SFB alleles. SFB8 was revealed to be the first known progenitor allele of a naturally occurring self-compatibility allele in Prunus, and consequently SC=The first intron of SC-RNase is a phase one intron, indicating its more recent evolutionary origin compared with the second intron. Sequence analysis of different cultivars revealed that more single nucleotide polymorphisms accumulated in SC-RNase than in SFBC. New methods were designed to allow high-throughput analysis of S genotypes of apricot cultivars and selections. S-RNase sequence data from various sources helped to elucidate the putative origin and dissemination of self-compatibility in apricot conferred by the SC haplotype.
在中国，杏（Prunus armeniaca）的起源中心，杏是自交不亲和的。然而，大多数欧洲品种是自交亲和的。在大多数情况下，自交亲和是SC单倍型中花粉基因（SFB）功能丧失突变的结果。本研究中进行的控制授粉表明，杂交组合‘Ceglédi óriás’（S8S9）בCeglédi arany’（SCS9）如预期那样坐果良好，但反交组合则不然。使用包括坐果评估、花粉管生长分析、核糖核酸酶活性测定、聚合酶链反应（PCR）分析以及S -核糖核酸酶和SFB等位基因的DNA测序在内的多层次方法，对杏的S8、S9和SC单倍型进行了分析。SFB8被揭示为李属中自然发生的自交亲和等位基因的首个已知祖先等位基因，因此SC = SC -核糖核酸酶的第一个内含子是一个1相内含子，表明其与第二个内含子相比具有更近的进化起源。不同品种的序列分析表明，SC -核糖核酸酶中积累的单核苷酸多态性比SFBC中更多。设计了新的方法以实现对杏品种和选系S基因型的高通量分析。来自各种来源的S -核糖核酸酶序列数据有助于阐明由SC单倍型赋予的杏自交亲和的假定起源和传播情况。
