卡他莫拉菌分子分型方法的比较
A comparison of molecular typing methods for Moraxella catarrhalis.
作者信息
Pingault N M, Lehmann D, Bowman J, Riley T V
机构信息
School of Biomedical, Biomolecular and Chemical Sciences, The University of Western Australia, Perth, WA, Australia.
出版信息
J Appl Microbiol. 2007 Dec;103(6):2489-95. doi: 10.1111/j.1365-2672.2007.03536.x.
AIMS
Three molecular typing techniques were examined to determine which method was the most discriminatory in order to perform epidemiological typing of Moraxella catarrhalis.
METHODS AND RESULTS
Twenty-five Mor. catarrhalis isolates obtained from nasopharyngeal aspirates collected from Aboriginal and non-Aboriginal children were subjected to random amplified polymorphic DNA (RAPD) analysis, automated ribotyping and pulsed field gel electrophoresis (PFGE). RAPD analysis determined two Mor. catarrhalis types, automated ribotyping with PstI determined four Mor. catarrhalis ribogroups and PFGE analysis with NotI determined 21 pulse field groups within the 25 isolates examined.
CONCLUSIONS
Analysis of discrimination index and typeability demonstrated that PFGE is the most discriminatory method for typing Mor. catarrhalis.
SIGNIFICANCE AND IMPACT OF THE STUDY
This study confirms that PFGE is the most appropriate molecular tool for the epidemiological study of Mor. catarrhalis.
目的
研究三种分子分型技术，以确定哪种方法在卡他莫拉菌的流行病学分型中具有最高的鉴别力。
方法与结果
从原住民和非原住民儿童鼻咽吸出物中分离得到25株卡他莫拉菌，对其进行随机扩增多态性DNA（RAPD）分析、自动核糖体分型和脉冲场凝胶电泳（PFGE）。RAPD分析确定了两种卡他莫拉菌类型，用PstI进行的自动核糖体分型确定了四个卡他莫拉菌核糖体组，用NotI进行的PFGE分析在25株受试菌株中确定了21个脉冲场组。
结论
鉴别指数和分型能力分析表明，PFGE是卡他莫拉菌分型中鉴别力最高的方法。
研究的意义和影响
本研究证实，PFGE是卡他莫拉菌流行病学研究最合适的分子工具。
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