随机扩增多态性DNA分析与脉冲场凝胶电泳用于卡他莫拉菌菌株分型的比较
Comparison of randomly amplified polymorphic DNA analysis and pulsed-field gel electrophoresis for typing of Moraxella catarrhalis strains.
作者信息
Vu-Thien H, Dulot C, Moissenet D, Fauroux B, Garbarg-Chenon A
机构信息
Service de Microbiologie, Hôpital d'Enfants Armand-Trousseau, 75571 Paris Cedex 12, France.
出版信息
J Clin Microbiol. 1999 Feb;37(2):450-2. doi: 10.1128/JCM.37.2.450-452.1999.
Abstract
Randomly amplified polymorphic DNA (RAPD) and pulsed-field gel electrophoresis (PFGE) for the analysis of 13 Moraxella catarrhalis isolates, 11 successive strains isolated from sputa of five children and 2 isolates obtained the same day from twins, were compared. RAPD and PFGE both yielded nine types from the 13 isolates, showing a chronic colonization with one strain in three patients and a successive colonization with different strains in two patients. The promising results obtained with RAPD should be confirmed with a larger number of strains, but RAPD seems as suitable as PFGE for the typing of M. catarrhalis.
摘要
比较了随机扩增多态性DNA（RAPD）和脉冲场凝胶电泳（PFGE）对13株卡他莫拉菌分离株的分析结果，其中11株连续菌株来自5名儿童的痰液，2株分离自同一天的双胞胎。RAPD和PFGE均从13株分离株中产生了9种类型，显示3名患者中有一种菌株的慢性定植，2名患者中有不同菌株的连续定植。用RAPD获得的有前景的结果应通过更多菌株进行验证，但RAPD似乎与PFGE一样适用于卡他莫拉菌的分型。
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