Mayta Holger, Hancock Kathy, Levine Min Z, Gilman Robert H, Farfán Marilú J, Verastegui Manuela, Lane William S, Garcia Hector H, Gonzalez Armando E, Tsang Victor C W
Department of International Health, Johns Hopkins Bloomberg School of Public Health, Baltimore, MD 21205, United States.
Mol Biochem Parasitol. 2007 Dec;156(2):154-61. doi: 10.1016/j.molbiopara.2007.07.017. Epub 2007 Aug 1.
Infections due to Taenia solium in humans (taeniasis/cysticercosis) remain a complex health problem, particularly in developing countries. We identified two oncosphere proteins that might protect the porcine intermediate host against cysticercosis and therefore help prevent disease in humans. One of these proteins was further identified by two-dimensional gel electrophoresis and micro-sequencing. The gene encoding this protective protein was also identified, cloned and characterized. The native 31.5 kDa protein Tso31 has four variants at the cDNA level. The longest sequence from which the others seem to derive, encodes a 253 amino acid peptide. The predicted protein has a molecular weight of 25.1 kDa, one putative N-glycosylation site, two fibronectin type III domains, and one C terminal transmembrane domain. The gene structure of the protein consists of four exons and three introns. The finding of one gene and four different cDNAs for Tso31 suggests the existence of a possible mechanism of differential splicing in this parasite. The Tso31 protein is exclusive to T. solium oncospheres with a putative protein structure of an extra-cellular receptor-like protein. The Tso31 protein was expressed as a recombinant protein fused to GST and tested in a vaccine to determine its effectiveness in protecting pigs against cysticercosis. Only two pigs out of eight vaccinated were protected and although the total median number of cyst decreased in vaccinated pigs compared to controls this decrease was not statistically significant (P = 0.09).
猪带绦虫在人类中引起的感染（猪带绦虫病/囊尾蚴病）仍然是一个复杂的健康问题，尤其是在发展中国家。我们鉴定出两种六钩蚴蛋白，它们可能保护猪中间宿主免受囊尾蚴病感染，从而有助于预防人类疾病。其中一种蛋白通过二维凝胶电泳和微量测序进一步鉴定。编码这种保护性蛋白的基因也被鉴定、克隆和表征。天然的31.5 kDa蛋白Tso31在cDNA水平上有四种变体。其他变体似乎从中衍生而来的最长序列编码一个253个氨基酸的肽。预测的蛋白分子量为25.1 kDa，有一个假定的N-糖基化位点、两个纤连蛋白III型结构域和一个C端跨膜结构域。该蛋白的基因结构由四个外显子和三个内含子组成。Tso31存在一个基因和四种不同cDNA的发现表明该寄生虫中可能存在可变剪接机制。Tso31蛋白是猪带绦虫六钩蚴特有的，具有类似细胞外受体蛋白的假定蛋白结构。Tso31蛋白被表达为与GST融合的重组蛋白，并在疫苗中进行测试，以确定其在保护猪免受囊尾蚴病感染方面的有效性。八只接种疫苗的猪中只有两只得到了保护，尽管与对照组相比，接种疫苗的猪体内囊肿的总中位数有所减少，但这种减少没有统计学意义（P = 0.09）。
