Mayta Holger, Hancock Kathy, Gilman Robert H, Zamudio Roxana, Castillo Janeth P, Levine Min Z, Garcia Hector H, Gonzalez Armando E, Tsang Victor C W
Departamento de Microbiologia, Facultad de Ciencias y Filosofia, Universidad Peruana Cayetano Heredia, Av. Honorio Delgado 430, San Martin de Porres, Lima 31, Peru.
J Parasitol. 2009 Jun;95(3):591-7. doi: 10.1645/GE-1767.1.
Taenia solium Linnaeus, 1758 is responsible for taeniasis and cysticercosis, which are 2 serious health problems, particularly in developing countries. The attempt to identify a 22.5kD possible protective oncospheral antigen by 2-dimensional gel-electrophoresis, micro-sequencing, and cDNA library screening produced a protein of 42kD that possesses a conserved domain similar to that of troponin T. Five variants that showed differences at the 5' end were observed at the cDNA level. Hyper-immune rabbit sera developed against recombinant GST fused protein identified the protein exclusively on activated oncospheres. The 42kD protein was tested in an enzyme-linked immunoassay (ELISA) alone and then together with the Tso31 protein for the diagnosis of human cysticercosis. When both antigens were combined, the test was found to be 85% sensitive and 65% specific. The 42kD is a novel T. solium protein that is present exclusively on activated oncospheres of this parasite, with poor diagnostic activity against taeniasis or human cysticercosis.
猪带绦虫（Taenia solium Linnaeus，1758）可引发猪带绦虫病和囊尾蚴病，这是两种严重的健康问题，在发展中国家尤为突出。通过二维凝胶电泳、微量测序和cDNA文库筛选来鉴定一种22.5kD可能的保护性六钩蚴抗原的尝试，得到了一种42kD的蛋白质，该蛋白质具有一个与肌钙蛋白T相似的保守结构域。在cDNA水平上观察到五个在5'端存在差异的变体。针对重组GST融合蛋白产生的超免疫兔血清仅在活化的六钩蚴上识别出该蛋白质。单独对42kD蛋白质进行酶联免疫吸附测定（ELISA）测试，然后与Tso31蛋白质一起用于诊断人类囊尾蚴病。当两种抗原结合使用时，该测试的灵敏度为85%，特异性为65%。42kD是一种新型的猪带绦虫蛋白质，仅存在于该寄生虫的活化六钩蚴上，对猪带绦虫病或人类囊尾蚴病的诊断活性较差。
