Santivañez S J, Hernández-González A, Chile N, Oleaga A, Arana Y, Palma S, Verastegui M, Gonzalez A E, Gilman R, Garcia H H, Siles-Lucas M
Cysticercosis Unit, Instituto Nacional de Ciencias Neurológicas, Lima, Peru.
Mol Biochem Parasitol. 2010 May;171(1):32-9. doi: 10.1016/j.molbiopara.2010.01.004. Epub 2010 Feb 6.
Taenia solium cysticerci are a major cause of human seizures and epilepsy in the world. In the gastrointestinal tract of infected individuals, taeniid eggs release the oncospheres, which are then activated by intestinal stimuli, getting ready to penetrate the gut wall and reach distant locations where they transform in cysticerci. Information about oncospheral molecules is scarce, and elucidation of the oncosphere proteome could help understanding the host-parasite relationship during the first steps of infection. In this study, using liquid chromatography and tandem mass spectrometry (LC-MS/MS) analysis, we could identify a set of oncospheral proteins involved in adhesion, protein folding, detoxification and proteolysis, among others. In addition, we have characterized one of the identified molecules, the parasite 14-3-3, by immunoblot and immunolocalization. The identification of these oncospheral proteins represents the first step to elucidate their specific roles in the biology of the host-parasite relationship.
猪带绦虫囊尾蚴是全球人类癫痫发作和癫痫的主要病因。在受感染个体的胃肠道中，带绦虫卵释放出六钩蚴，随后六钩蚴被肠道刺激激活，准备穿透肠壁并到达远处，在那里它们会转变为囊尾蚴。关于六钩蚴分子的信息很少，阐明六钩蚴蛋白质组有助于理解感染初期的宿主-寄生虫关系。在本研究中，我们使用液相色谱和串联质谱（LC-MS/MS）分析，鉴定出一组参与黏附、蛋白质折叠、解毒和蛋白水解等过程的六钩蚴蛋白。此外，我们通过免疫印迹和免疫定位对其中一个已鉴定的分子——寄生虫14-3-3进行了表征。这些六钩蚴蛋白的鉴定是阐明它们在宿主-寄生虫关系生物学中具体作用的第一步。
