Genetics Department, Faculty of Agriculture, Zagazig University, Zagazig 44511, Egypt.
Department of Biochemistry, Faculty of Sciences, University of Tabuk, Tabuk 71491, Saudi Arabia.
Molecules. 2022 May 1;27(9):2895. doi: 10.3390/molecules27092895.
In the present study, the chemical composition and total phenolic (TPC) and total flavonoid contents (TFC) of eight soybean cultivars (Giza 21, Giza 22, Giza 35, Giza 111, Giza 82, Giza 83, Crawford, and Holliday) were estimated. Moreover, antioxidant activity and in vitro cytotoxic activities against HepG-2 and MCF-7 were evaluated. Giza 21, Giza 111, and Crawford cultivars recorded higher than 40% crude protein. The analysis revealed that TPC values in seed extracts ranged from 10.5 mg GAE/g extract in Giza 35 to 6.4 mg GAE/g extract in Giza 22. TFC varied from 1.20 mg QE/g extract in Giza 111 to 0.55 mg QE/g extract in Crawford. Giza 35 exhibited the highest content of genistein and daidzein and the highest free radical scavenging activity (61.833%). The results of the MTT assay demonstrated that the soybean methanolic extracts inhibited the proliferation of HepG-2 and MCF-7 cells in a dose-dependent manner. Giza 35 exhibited the highest cytotoxic activity. In conclusion, Giza 35 cultivar recorded the highest TPC and TFC values and antioxidant and cytotoxic activities. Therefore, this cultivar can be used as a source for the production of pharmaceutical and medicinal products rather than as a nutritional source of protein.
本研究测定了 8 个大豆品种（Giza 21、Giza 22、Giza 35、Giza 111、Giza 82、Giza 83、Crawford 和 Holliday）的化学成分以及总酚（TPC）和总类黄酮含量（TFC）。此外，还评估了抗氧化活性和对 HepG-2 和 MCF-7 的体外细胞毒性。Giza 21、Giza 111 和 Crawford 品种的粗蛋白含量高于 40%。分析表明，种子提取物中的 TPC 值范围从 Giza 35 的 10.5mgGAE/g 提取物到 Giza 22 的 6.4mgGAE/g 提取物。TFC 从 Giza 111 的 1.20mgQE/g 提取物到 Crawford 的 0.55mgQE/g 提取物不等。Giza 35 表现出最高的染料木素和大豆苷元含量以及最高的自由基清除活性（61.833%）。MTT 测定结果表明，大豆甲醇提取物以剂量依赖的方式抑制 HepG-2 和 MCF-7 细胞的增殖。Giza 35 表现出最高的细胞毒性。总之，Giza 35 品种具有最高的 TPC 和 TFC 值以及抗氧化和细胞毒性活性。因此，该品种可作为生产药物和医药产品的来源，而不是蛋白质的营养来源。
