Baran Wojciech, Szepietowski Jacek C, Mazur Grzegorz, Baran Eugeniusz
Department of Dermatology, Venereology and Allergology, Wroclaw Medical University, Wroclaw, Poland.
Biomarkers. 2008 Feb;13(1):52-8. doi: 10.1080/13547500701610273.
This study was performed to investigate the association between interferon (IFN)-gamma single nucleotide polymorphism (SNP) and susceptibility for psoriasis vulgaris. DNA from 78 patients with psoriasis vulgaris (54 patients with type I psoriasis, 24 with type II psoriasis) and 74 healthy volunteers was investigated. IFN-gamma promoter gene SNP in position 874 was evaluated by polymerase chain reaction with sequence-specific primers (PCR-SSP) and the results were compared between a group of psoriatic patients, divided into early onset of psoriasis (type I) and late onset of psoriasis (type II) subgroups, and healthy control subjects. A significant difference in the genotype frequencies between psoriasis patients and healthy controls was found (p < 0.02) and no significant differences were observed analyzing subsets of psoriatic patients (gender, type of disease) also in carriage and allele frequencies. The results suggest that IFN-gamma polymorphism is associated with susceptibility to psoriasis vulgaris.
本研究旨在探讨干扰素（IFN）-γ单核苷酸多态性（SNP）与寻常型银屑病易感性之间的关联。对78例寻常型银屑病患者（54例Ⅰ型银屑病患者，24例Ⅱ型银屑病患者）及74名健康志愿者的DNA进行了研究。采用序列特异性引物聚合酶链反应（PCR-SSP）评估874位的IFN-γ启动子基因SNP，并将一组银屑病患者（分为银屑病早发型（Ⅰ型）和晚发型（Ⅱ型）亚组）与健康对照者的结果进行比较。发现银屑病患者与健康对照者的基因型频率存在显著差异（p < 0.02），分析银屑病患者亚组（性别、疾病类型）的携带率和等位基因频率时未观察到显著差异。结果表明，IFN-γ多态性与寻常型银屑病易感性相关。
