Popadic Svetlana, Savic Emina, Markovic Milos, Ramic Zorica, Medenica Ljiljana, Pravica Vera, Spuran Zorica, Trajkovic Vladimir, Popadic Dusan
Department of Dermatovenereology, School of Medicine, University of Belgrade, Serbia. ; Clinic of Dermatovenereology, Clinical Center of Serbia, Serbia.
Institute of Microbiology and Immunology, School of Medicine, University of Belgrade, Serbia.
Ann Dermatol. 2015 Apr;27(2):128-32. doi: 10.5021/ad.2015.27.2.128. Epub 2015 Mar 24.
Psoriasis is a common chronic inflammatory skin disease with a strong genetic basis. Cytokines such as tumor necrosis factor alpha (TNF-α), interleukins (ILs) such are IL-12 and IL-23, and interferon gamma (IFN-γ) are released from various inflammatory and resident cells, and have been implicated in the initiation/maintenance of inflammation. Certain alleles of the aforementioned cytokines may be associated with disease susceptibility/severity.
To investigate the association of three common functional gene polymorphisms, namely TNF -308 G/A (rs1800629), IL12B (encoding the p40 subunit of IL-12/23) +1188 A/C (rs3212227), and IFNG +874 T/A (rs2430561) with psoriasis development and severity in Serbian patients.
We genotyped 130 patients with psoriasis (26 of whom also had psoriatic arthritis) and 259 controls; rs1800629 and rs3212227, and rs2430561, by real-time PCR assay.
The TNF GG genotype was detected at a higher frequency in patients with psoriasis compared to control subjects (OR, 1.420; 95% CI, 0.8702.403) without statistical significance (p=0.191). Lack of the TNF G allele was associated with lower psoriasis severity (p=0.007). The IL12B AC genotype was underrepresented in the patients with psoriatic arthritis compared to healthy subjects (OR, 0.308; 95% CI, 0.0901.057; p=0.049). The distribution of the rs2430561 allele and genotype frequencies was similar between patients with psoriasis and controls.
Our study demonstrates an effect of the rs1800629 on psoriasis severity, and a marginal impact of the rs3212227 on susceptibility to psoriatic arthritis. Collectively, our results obtained in a Serbian cohort expand current knowledge regarding individual predisposition to psoriatic disease.
银屑病是一种常见的具有强大遗传基础的慢性炎症性皮肤病。细胞因子如肿瘤坏死因子α（TNF-α）、白细胞介素（ILs）如IL-12和IL-23以及干扰素γ（IFN-γ）从各种炎症细胞和驻留细胞中释放出来，并与炎症的起始/维持有关。上述细胞因子的某些等位基因可能与疾病易感性/严重程度相关。
研究三种常见的功能基因多态性，即TNF -308 G/A（rs1800629）、IL12B（编码IL-12/23的p40亚基）+1188 A/C（rs3212227）和IFNG +874 T/A（rs2430561）与塞尔维亚患者银屑病发生及严重程度的相关性。
我们对130例银屑病患者（其中26例还患有银屑病关节炎）和259例对照进行基因分型；通过实时聚合酶链反应检测rs1800629、rs3212227和rs2430561。
与对照相比，银屑病患者中TNF GG基因型的检测频率更高（比值比，1.420；95%可信区间，0.8702.403），但无统计学意义（p = 0.191）。缺乏TNF G等位基因与较低的银屑病严重程度相关（p = 0.007）。与健康受试者相比，银屑病关节炎患者中IL12B AC基因型的比例较低（比值比，0.308；95%可信区间，0.0901.057；p = 0.049）。银屑病患者和对照之间rs2430561等位基因和基因型频率的分布相似。
我们的研究证明rs1800629对银屑病严重程度有影响，rs3212227对银屑病关节炎易感性有轻微影响。总体而言，我们在塞尔维亚队列中获得的结果扩展了目前关于银屑病个体易感性的知识。
