Department of Pharmacognosy, Faculty of Pharmacy, Gazi University, Etiler, 06330 Ankara, Turkey.
Department of Pharmacognosy, Faculty of Pharmacy, Ankara University, 06100 Ankara, Turkey.
Evid Based Complement Alternat Med. 2014;2014:578103. doi: 10.1155/2014/578103. Epub 2014 Aug 3.
The aim of the present research is to evaluate the antiurolithiatic effect of the various extracts prepared from the fruits of Viburnum opulus L., in regard to its ethnobotanical record. To induce urolithiasis, 70 mg/kg sodium oxalate was injected to the rats which were housed individually in metabolic cages. The test materials were applied during 7 days. Biochemical (urine and serum parameters), histopathological and antioxidant (TBARs, TSH and GSH) assays were conducted. The urine samples were examined by light microscope for the determination of the calcium oxalate crystals. Lyophilized juice of V. opulus (LJVO) and lyophilized commercial juice of V. opulus (LCJVO) exerted potential antiurolithiatic activity which was attributed to its diuretic effect along with the inhibitory action on the oxalate levels and free radical production. We also determined the chlorogenic acid content of the LJVO by high-performance liquid chromatography (HPLC). Chlorogenic acid was determined by using Supelcosil LC-18 (250 × 4.6 mm, 5 µm) column and acetonitrile: water: 0.2% o-phosphoric acid as a mobile phase. The chlorogenic acid content of V. opulus was found to be 0.3227 mg/mL in fruit juice. The results obtained in this study have provided a scientific evidence for the traditional usage of V. opulus on passing kidney stones in Turkish folk medicine.
本研究旨在评估从欧洲荚蒾果实中制备的各种提取物的抗结石作用，以其民族植物学记录为依据。为了诱导结石形成，将 70mg/kg 的草酸钠注射到单独饲养在代谢笼中的大鼠体内。在 7 天内应用测试材料。进行了生化（尿液和血清参数）、组织病理学和抗氧化（TBARs、TSH 和 GSH）测定。通过光显微镜检查尿液样本以确定草酸钙晶体。欧洲荚蒾冻干汁（LJVO）和欧洲荚蒾冻干商业汁（LCJVO）表现出潜在的抗结石活性，这归因于其利尿作用以及对草酸盐水平和自由基生成的抑制作用。我们还通过高效液相色谱法（HPLC）测定了 LJVO 中的绿原酸含量。使用 Supelcosil LC-18（250×4.6mm，5μm）柱和乙腈：水：0.2%邻磷酸作为流动相测定绿原酸。在果汁中发现欧洲荚蒾的绿原酸含量为 0.3227mg/mL。本研究的结果为土耳其民间医学中使用欧洲荚蒾通过肾脏排石的传统用途提供了科学依据。
