Vestergaard T A, Nielsen S L, Dahlerup J F, Hornung N
Department of Clinical Biochemistry, Randers Regional Hospital, Randers, Denmark.
Scand J Clin Lab Invest. 2008;68(4):343-7. doi: 10.1080/00365510701576198.
Calprotectin, a protein found mainly in neutrophil granulocytes, is used as an inflammatory marker, while the fecal concentration of the protein is used to detect gastrointestinal (GI) inflammation.
Fecal calprotectin in 100 stool samples was measured by the ELISA method and by a new rapid test. Eighty-two patients had fecal calprotectin measured for clinical reasons and delivered 95 stool samples. The rest were delivered by healthy volunteers.
The association between the two tests was statistically significant (p<0.0001, chi(2) test). With calprotectin values <15 microg/g, the sensitivity and specificity of the new rapid test was 96 % (95 % confidence interval (CI), 87-100 %) and 70 % (CI, 55-83 %), respectively, with a negative predictive value of 94 % (CI, 81-99 %). With values >15 microg/g, the rapid test was less accurate, thus rendering results in this range difficult to interpret.
The new rapid test is useful as a screening test for excluding GI inflammation when the cut-off of 15 microg/g is used. With fecal calprotectin concentrations >15 microg/g, the rapid test should be supplemented by quantitative measurement.
钙卫蛋白是一种主要存在于中性粒细胞中的蛋白质，用作炎症标志物，而该蛋白的粪便浓度用于检测胃肠道（GI）炎症。
采用酶联免疫吸附测定（ELISA）法和一种新的快速检测法对100份粪便样本中的粪便钙卫蛋白进行检测。82例患者因临床原因检测粪便钙卫蛋白，提供了95份粪便样本。其余样本由健康志愿者提供。
两种检测方法之间的关联具有统计学意义（p<0.0001，卡方检验）。当钙卫蛋白值<15μg/g时，新快速检测法的灵敏度和特异性分别为96%（95%置信区间（CI），87-100%）和70%（CI，55-83%），阴性预测值为94%（CI，81-99%）。当值>15μg/g时，快速检测法准确性较低，因此该范围内的结果难以解释。
当使用15μg/g的临界值时，新的快速检测法作为排除胃肠道炎症的筛查检测方法很有用。当粪便钙卫蛋白浓度>15μg/g时，快速检测法应辅以定量检测。
