Mak S, Mak I, Gallie B L, Godbout R, Phillips R A
Int J Cancer. 1982 Dec 15;30(6):697-700. doi: 10.1002/ijc.2910300604.
Retinoblastoma is a rare eye tumor found predominantly in young children. Intra-ocular injection of adenovirus-12 (Ad-12) into newborn recipients induces in rats and in baboons tumors morphologically similar to human retinoblastoma tumors. In addition, Ad-12 DNA can transform human embryonic retinal cells and the transformed cells form retinoblastoma-like tumors in nude mice. These observations have led to the suggestion that Ad-12 may also be involved in the induction of human retinoblastoma tumors. To test this possibility, DNA and RNA were isolated from six human tumors. Analysis of RNA and DNA by blotting with labelled Ad-12 failed to reveal viral sequences in human retinoblastoma.
视网膜母细胞瘤是一种主要在幼儿中发现的罕见眼部肿瘤。将腺病毒12（Ad - 12）眼内注射到新生受体中，可在大鼠和狒狒体内诱发形态上与人类视网膜母细胞瘤相似的肿瘤。此外，Ad - 12 DNA可转化人类胚胎视网膜细胞，且转化后的细胞在裸鼠体内形成视网膜母细胞瘤样肿瘤。这些观察结果提示Ad - 12可能也参与了人类视网膜母细胞瘤的诱发过程。为了验证这种可能性，从6例人类肿瘤中分离出DNA和RNA。用标记的Ad - 12进行印迹分析RNA和DNA，未能在人类视网膜母细胞瘤中发现病毒序列。
