Wang Lei, Chen Jing-Tang
College of Agronomy, Hebei Agricultural University, Baoding 071001, China.
Yi Chuan. 2007 Sep;29(9):1055-60. doi: 10.1360/yc-007-1055.
With the completion of the model plant genome sequencing projects such as Arabidopsis and rice (Oryza sativa L.), comparative genomics has been developed rapidly in recent years. As a new branch of science, this technique has offered a new approach for studying plant genome evolution, structure and function. In this paper, the research strategies, such as comparative genetic mapping, microcollinearity study in gene structure, comparative research on the ESTs and proteins, comparative QTL mapping among crops, and comparative map-based cloning of genes, were summarized. We reviewed the principles, characteristics, feasibilities and progresses of various strategies that were used in plant comparative genomics. It would be helpful to use the sequence data from model plants to elucidate the structure and function of other crop genomes.
随着拟南芥和水稻等模式植物基因组测序项目的完成，近年来比较基因组学发展迅速。作为一门新的科学分支，该技术为研究植物基因组的进化、结构和功能提供了一种新方法。本文总结了比较遗传图谱构建、基因结构的微共线性研究、ESTs和蛋白质的比较研究、作物间比较QTL定位以及基于比较图谱的基因克隆等研究策略。我们综述了植物比较基因组学中使用的各种策略的原理、特点、可行性和进展。利用模式植物的序列数据来阐明其他作物基因组的结构和功能将是有益的。
