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来自假单胞菌菌株CRL-OK的氨基甲酸甲酯水解酶的纯化与特性分析

Purification and characterization of the N-methylcarbamate hydrolase from Pseudomonas strain CRL-OK.

作者信息

Mulbry W W, Eaton R W

机构信息

Pesticide Degradation Laboratory, Agricultural Research Service, Beltsville, Maryland 20705.

出版信息

Appl Environ Microbiol. 1991 Dec;57(12):3679-82. doi: 10.1128/aem.57.12.3679-3682.1991.

DOI:10.1128/aem.57.12.3679-3682.1991
PMID:1785941
原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC184037/
Abstract

A unique cytosolic enzyme that hydrolyzes the carbamate linkage of the insecticide carbaryl (1-naphthyl N-methylcarbamate) was purified from extracts of Pseudomonas sp. strain CRL-OK. Substrates of the hydrolase include the N-methylcarbamate pesticides carbofuran and aldicarb but not the phenylcarbamate isopropyl m-chlorocarbanilate, the thiocarbamate S-ethyl N,N-dipropylthiocarbamate, or the dimethylcarbamate o-nitrophenyldimethylcarbamate.

摘要

从假单胞菌属菌株CRL-OK的提取物中纯化出一种独特的胞质酶，该酶可水解杀虫剂西维因（1-萘基N-甲基氨基甲酸酯）的氨基甲酸酯键。水解酶的底物包括N-甲基氨基甲酸酯类农药克百威和涕灭威，但不包括苯基氨基甲酸酯类间氯苯胺基异丙酯、硫代氨基甲酸酯类S-乙基N,N-二丙基硫代氨基甲酸酯或二甲基氨基甲酸酯类邻硝基苯基二甲基氨基甲酸酯。

https://cdn.ncbi.nlm.nih.gov/pmc/blobs/d047/184037/a46811a295c0/aem00065-0290-a.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/d047/184037/a46811a295c0/aem00065-0290-a.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/d047/184037/a46811a295c0/aem00065-0290-a.jpg

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Purification and Characterization of Carbaryl Hydrolase from Blastobacter sp. Strain M501.从 Blastobacter sp. 菌株 M501 中纯化和表征甲萘威水解酶。
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