Dick J W, Johnson J W
Avian Dis. 1985 Jul-Sep;29(3):706-14.
An enzyme-linked immunosorbent assay (ELISA) was developed to detect antibodies in broiler breeders vaccinated (wing web) with the CU fowl cholera vaccine. Birds were bled weekly from 9 to 26 weeks, every other week from 26 to 40 weeks, and every 4 weeks from 40 to 56 weeks of age. Overall mean ELISA antibody titers (9 to 56 weeks) reported as log10 values and survivability of the vaccinates after virulent challenge were as follows: unvaccinated--5.75, 48%; birds vaccinated once at 8 weeks--5.91, 78%; birds vaccinated twice at 8 and 14 weeks--6.11, 100%; birds vaccinated thrice at 8, 14, and 20 weeks--6.23, 100%; birds vaccinated twice at 8 and 20 weeks--6.12, 100%; and birds vaccinated twice at 8 and 20 weeks (plus fowl pox at 8 weeks)--6.08, 95%. Survivability in the vaccinates after virulent challenge with strain X-73 Pasteurella multocida was 100% in birds with ELISA antibody titers (log10) greater than 6.02. Under the conditions of this experiment, birds vaccinated two or three times between 8 and 20 weeks proved to be sufficiently immune at 56 weeks of age to withstand a virulent fowl cholera challenge. Birds not vaccinated or vaccinated only once at 8 weeks were not sufficiently immunized to withstand virulent challenge.
开发了一种酶联免疫吸附测定（ELISA）来检测用CU禽霍乱疫苗（翼膜接种）免疫的肉种鸡中的抗体。从9至26周龄每周采集鸡的血液，从26至40周龄每两周采集一次，从40至56周龄每四周采集一次。报告的总体平均ELISA抗体滴度（9至56周）以log10值表示，强毒攻击后接种疫苗鸡的存活率如下：未接种疫苗的鸡——5.75，48%；8周龄接种一次疫苗的鸡——5.91，78%；8周龄和14周龄接种两次疫苗的鸡——6.11，100%；8周龄、14周龄和20周龄接种三次疫苗的鸡——6.23，100%；8周龄和20周龄接种两次疫苗的鸡——6.12，100%；8周龄和20周龄接种两次疫苗（8周龄时还接种了鸡痘疫苗）的鸡——6.08，95%。用多杀性巴氏杆菌X-73菌株进行强毒攻击后，ELISA抗体滴度（log10）大于6.02的接种疫苗鸡的存活率为100%。在本实验条件下，8至20周龄接种两次或三次疫苗的鸡在56周龄时被证明具有足够的免疫力，能够抵御强毒禽霍乱攻击。未接种疫苗或8周龄仅接种一次疫苗的鸡未获得足够的免疫，无法抵御强毒攻击。
