Elwing H, Tengvall P, Askendal A, Lundström I
Laboratory of Applied Physics, Linköping University, Sweden.
J Biomater Sci Polym Ed. 1991;3(1):7-15. doi: 10.1163/156856292x00042.
The exchange sequence of plasma proteins in narrow spaces on solid surfaces was studied by means of a modified 'lens-on-surface' method as originally described by Vroman and Adams. In our studies, lateral scanning ellipsometry was used as the detection method. With the use of antibodies it was demonstrated and confirmed that immunologically detectable plasma protein antigens appear and disappear in a time- and concentration-dependent sequence [IgG followed by fibrinogen followed by high-molecular weight kininogen (HMWK)] on silica surfaces. Plasma protein exchange reactions were also studied on hydrophilic titanium (Ti), vanadium (V), and silver (Ag) surfaces. Atypical exchange patterns were found on V and Ag surfaces as compared with hydrophilic silica (adsorbed fibrinogen was not removed).
采用最初由弗罗曼和亚当斯描述的改良“表面透镜”方法，研究了固体表面狭窄空间内血浆蛋白的交换序列。在我们的研究中，使用横向扫描椭圆偏振仪作为检测方法。通过使用抗体，证实并确认了免疫可检测的血浆蛋白抗原在二氧化硅表面以时间和浓度依赖性序列出现和消失[免疫球蛋白G（IgG）之后是纤维蛋白原，然后是高分子量激肽原（HMWK）]。还研究了亲水性钛（Ti）、钒（V）和银（Ag）表面的血浆蛋白交换反应。与亲水性二氧化硅相比，在钒和银表面发现了非典型的交换模式（吸附的纤维蛋白原未被去除）。
