Zhang M, Desai T, Ferrari M
Biomedical Microdevices Center, College of Engineering, University of California, USA.
Biomaterials. 1998 May;19(10):953-60. doi: 10.1016/s0142-9612(98)00026-x.
Silicon surfaces were modified by covalent attachment of a self-assembled (SA) polyethylene glycol (PEG) film. Adsorption of albumin, fibrinogen, and IgG to PEG immobilized silicon surfaces was studied by ellipsometry to evaluate the non-fouling and non-immunogenic properties of the surfaces. The adhesion and proliferation of human fibroblast and Hela cells onto the modified surfaces were investigated to examine their tissue biocompatibility. Coated PEG chains showed the effective depression of both plasma protein adsorption and cell attachment to the modified surfaces. The mechanisms accounting for the reduction of protein adsorption and cell adhesion on modified surfaces were discussed.
通过共价连接自组装（SA）聚乙二醇（PEG）膜对硅表面进行改性。采用椭偏仪研究了白蛋白、纤维蛋白原和IgG在固定有PEG的硅表面的吸附情况，以评估该表面的抗污染和非免疫原性特性。研究了人成纤维细胞和Hela细胞在改性表面上的黏附与增殖情况，以考察其组织生物相容性。包覆的PEG链有效抑制了血浆蛋白在改性表面的吸附以及细胞的附着。探讨了改性表面上蛋白质吸附和细胞黏附减少的机制。
