Denenberg V H, Mobraaten L E, Sherman G F, Morrison L, Schrott L M, Waters N S, Rosen G D, Behan P O, Galaburda A M
Biobehavioral Sciences Graduate Degree Program, University of Connecticut, Storrs 06269-4154.
Brain Res. 1991 Nov 1;563(1-2):114-22. doi: 10.1016/0006-8993(91)91522-3.
NZB and BXSB mice develop autoimmune disease and learn poorly on avoidance tasks. In addition, many of these mice have ectopic collections of neurons, which occur prenatally, in layer I of the cerebral neocortex. The purpose of these experiments was to evaluate the contribution of the uterine/maternal environment upon these variables by transferring fertilized ova to an autoimmune or a non-autoimmune maternal host. In Experiment 1 fertilized DBA ova were transferred into the uteri of BXSB maternal recipients. Later, these animals and conventionally reared DBAs were tested for paw preference, swimming rotation, water escape learning, and shuttlebox avoidance learning. Blood was taken for measurement of immune parameters, and their brains were examined for cortical ectopias. As compared to conventional DBAs, the ova transfer mice had greater amounts of anti-dsDNA autoantibodies, poorer avoidance learning, and poorer water escape learning; in addition, the females had greater paw asymmetry. There was only 1 ectopia in the 81 ova transfer animals, and none in the 78 control mice. In Experiment 2 fertilized NZB ova were transferred into the uteri of non-autoimmune hybrid females and the same procedures were followed as in Experiment 1. Ova transfer mice had lesser amounts of anti-dsDNA autoantibodies, better avoidance learning scores, and females had less paw asymmetry; in addition, within the ova transfer group males were clockwise swimmers whereas females swam counterclockwise. There were 4 ectopics out of 17 ova transfer mice (23.5%), which did not differ from the 40.5% of the control group. In both experiments the uterine environment did not affect the occurrence of ectopias.(ABSTRACT TRUNCATED AT 250 WORDS)
NZB和BXSB小鼠会患上自身免疫性疾病，并且在回避任务中的学习能力较差。此外，许多这类小鼠在大脑新皮质的I层有神经元的异位聚集，这种情况发生在产前。这些实验的目的是通过将受精卵转移到自身免疫或非自身免疫的母体宿主中，来评估子宫/母体环境对这些变量的影响。在实验1中，将受精的DBA卵转移到BXSB母体受体的子宫中。之后，对这些动物和常规饲养的DBA小鼠进行爪偏好、游泳旋转、水逃避学习和穿梭箱回避学习测试。采集血液测量免疫参数，并检查它们的大脑是否有皮质异位。与常规DBA小鼠相比，卵转移小鼠有更多的抗双链DNA自身抗体、更差的回避学习能力和水逃避学习能力；此外，雌性小鼠的爪不对称性更大。在81只卵转移动物中只有1个异位，而78只对照小鼠中没有。在实验2中，将受精的NZB卵转移到非自身免疫杂种雌性的子宫中，并遵循与实验1相同的程序。卵转移小鼠的抗双链DNA自身抗体数量较少，回避学习分数较好，雌性小鼠的爪不对称性较小；此外，在卵转移组中，雄性小鼠顺时针游泳，而雌性小鼠逆时针游泳。17只卵转移小鼠中有4只（23.5%）有异位，这与对照组的40.5%没有差异。在两个实验中，子宫环境均未影响异位的发生。（摘要截断于250字）
