犬甲状腺球蛋白的特性及放射免疫测定

Characterization of and radioimmunoassay for canine thyroglobulin.

作者信息

Verschueren C P, Selman P J, de Vijlder J J, Mol J A

机构信息

Department of Clinical Sciences of Companion Animals, University of Utrecht, The Netherlands.

出版信息

Domest Anim Endocrinol. 1991 Oct;8(4):509-19. doi: 10.1016/0739-7240(91)90020-k.

DOI:10.1016/0739-7240(91)90020-k

PMID:1786700

Abstract

Canine thyroglobulin (cTg) has been isolated and purified. It has similar electrophoretic patterns as Tg from other mammalian species. The main fraction had a MW of 660,000, whereas also fractions of a MW of approximately 1,300,000 (dimer) and 330,000 (subunit) were present. The iodine content was 0.8 to 1.0% (w/w). cTg did not cross-react with antibodies against human Tg to a degree that would allow the use of a radioimmunoassay for human Tg for the determination of cTg in serum or plasma. Therefore a polyclonal antiserum was raised against cTg and a homologous radioimmunoassay was developed, which was sensitive (0.4 micrograms/l) and specific (cross-reactivity in cTg assay of human Tg, goat Tg, T4, T3, and DIT less than 0.01%). Plasma Tg levels in normal dogs of both sexes and aged 3-15 years amounted to 192 +/- 73 micrograms/l (mean +/- SD, n = 30). There was no relation between plasma Tg and T4 levels.

摘要

犬甲状腺球蛋白（cTg）已被分离和纯化。它具有与其他哺乳动物物种的甲状腺球蛋白（Tg）相似的电泳图谱。主要组分的分子量为660,000，同时也存在分子量约为1,300,000（二聚体）和330,000（亚基）的组分。碘含量为0.8至1.0%（w/w）。cTg与抗人Tg抗体的交叉反应程度不允许使用人Tg放射免疫测定法来测定血清或血浆中的cTg。因此，制备了抗cTg的多克隆抗血清并开发了同源放射免疫测定法，该方法灵敏（0.4微克/升）且特异（人Tg、山羊Tg、T4、T3和二碘酪氨酸在cTg测定中的交叉反应率小于0.01%）。3至15岁的正常雌雄犬血浆Tg水平为192±73微克/升（平均值±标准差，n = 30）。血浆Tg与T4水平之间无关联。