Liu Li, Geisert Eldon E, Frankfurter Anthony, Spano Anthony J, Jiang Chloe Xue, Yue Junming, Dragatsis Ioannis, Goldowitz Dan
Department of Anatomy and Neurobiology, University of Tennessee Health Science Center, Memphis, Tennessee 38163, USA.
Genesis. 2007 Sep;45(9):560-9. doi: 10.1002/dvg.20325.
A yellow fluorescence protein (YFP) reporter construct was cloned downstream of the beta-tubulin III promoter and injected to produce two founder lines of transgenic mice. YFP expression was observed in many regions of the developing peripheral and central nervous system. YFP expression was first observed in the peripheral and central nervous system as early as embryonic day 9.0. There was a dramatic increase in the number of neuronal systems expressing YFP through P0. Then as the animals reached adult age, the expression levels decreased, but many neurons still show YFP expression, notably in regions of the brain undergoing adult neurogenesis, i.e., the rostral migratory stream and subgranular layer of the dentate gyrus. This reporter-based staining was compared with anti-class-III beta-tubulin immunocytochemistry and shown to closely parallel the expression of the endogenous protein. These transgenic lines should provide unique models to study in vivo and in vitro neurodevelopment.
将黄色荧光蛋白（YFP）报告基因构建体克隆到β-微管蛋白III启动子下游，并进行注射以产生两个转基因小鼠创始系。在发育中的外周和中枢神经系统的许多区域观察到YFP表达。早在胚胎第9.0天就在外周和中枢神经系统中首次观察到YFP表达。通过出生后第0天，表达YFP的神经元系统数量急剧增加。然后随着动物成年，表达水平下降，但许多神经元仍显示YFP表达，特别是在经历成年神经发生的脑区，即齿状回的吻侧迁移流和颗粒下层。将这种基于报告基因的染色与抗III类β-微管蛋白免疫细胞化学进行比较，结果表明其与内源性蛋白的表达密切平行。这些转基因系应该为体内和体外神经发育研究提供独特模型。
