Oleszczuk Agnieszka, Spannbauer Michael, Tannapfel Andrea, Blüher Matthias, Hengstler Jan, Pietsch Uta-Carolin, Schuhmacher Annette, Wittekind Christian, Hauss Johann P, Schön Michael R
Department of Surgery, University of Leipzig, Liebigstr. 20, 04103 Leipzig, Germany.
Exp Toxicol Pathol. 2007 Nov;59(3-4):205-13. doi: 10.1016/j.etp.2007.05.009. Epub 2007 Sep 14.
Independent of etiology, the hepatic microvesicular steatosis has a worse prognosis compared with macrovesicular steatosis. Proliferation compensates for apoptosis and reflects regenerative mechanisms following liver injury. It is unknown whether these two types of fatty liver have differences in regenerative capacity and apoptosis, which could have an impact on their prognosis.
Two groups of pigs were studied for 72 days under a protein-deficient diet. One group received only protein-deficient diet (n=6), the other was treated in addition to the diet with 6g ethanol/kg/day by means of a percutaneous intragastric catheter (n=6). The rate of proliferating and apoptotic hepatocytes was determined, respectively, by proliferation cell nuclear antigen (PCNA) and ISEL/TUNEL staining for apoptosis in liver biopsies with similar steatosis grade in pigs with micro- or macrovesicular fatty liver.
The ethanol-treated group developed microvesicular steatosis, the other group developed macrovesicular steatosis. Proliferation index was significantly increased in macrovesicular in comparison with microvesicular steatosis (p<0.05). Apoptosis rate was similar in both groups.
Regeneration, but not apoptosis rate differs between micro- and macrovesicular steatosis. The reduced regenerative capacity in microvesicular steatosis may contribute to the worse prognosis of this subtype of fatty liver disease.
无论病因如何，肝微泡性脂肪变性的预后比大泡性脂肪变性更差。增殖可补偿凋亡，并反映肝损伤后的再生机制。这两种类型的脂肪肝在再生能力和凋亡方面是否存在差异尚不清楚，而这可能会影响它们的预后。
两组猪在蛋白质缺乏饮食条件下研究72天。一组仅接受蛋白质缺乏饮食（n = 6），另一组除饮食外，通过经皮胃内导管给予6g乙醇/ kg/天（n = 6）。分别通过增殖细胞核抗原（PCNA）和ISEL/TUNEL染色测定微泡性或大泡性脂肪肝且脂肪变性程度相似的猪肝脏活检中增殖和凋亡肝细胞的比率。
乙醇处理组出现微泡性脂肪变性，另一组出现大泡性脂肪变性。与微泡性脂肪变性相比，大泡性脂肪变性的增殖指数显著增加（p<0.05）。两组的凋亡率相似。
微泡性和大泡性脂肪变性之间再生能力不同，但凋亡率无差异。微泡性脂肪变性中再生能力降低可能导致这种亚型脂肪肝疾病的预后更差。
