Ou H-Y, Chao P-H, Yu P-C, Wei Y-C, Chen C-L, Yu C-Y, Chiu T-M, Chang Y-C, Lai C-Y, Cheng Y-F
Department of Diagnostic Radiology, Kaohsiung Chang Gung Memorial Hospital, Chang Gung University College of Medicine, Kaohsiung, Taiwan.
Transplant Proc. 2012 May;44(4):955-8. doi: 10.1016/j.transproceed.2012.03.026.
Hepatic steatosis (HE), which is common among the general population, is present in donor organs, potentially affecting their graft survival as well as the recovery of the donor. Our goal was to develop an experimentally and clinically reliable, noninvasive method to quantify macrovesicular and microvesicular hepatic steatosis using 3-T (1)H-magnetic resonance spectroscopy (MRS).
Macrovesicular and microvescular steatosis were induced in rats using methylcholine deficiency and choline deficiency diets. A MayoBC10 coil was used for radiofrequency transmission and signal recept. Measurements of hepatic fat content were performed using (1)H spectroscopy on a 3.0-T whole-body GE Signa system. The ratio of the areas under the curve of fat (0.8-1.3 ppm) and water (4.7 ppm) was used to determine hepatic fat content, which was compared with the degree of histopathologic and biochemical steatosis.
Twenty rats were divided into two groups based on the percentage of microvesicular liver steatosis. Group A (n = 13) was the lower percentage group (microvesicular < 10%) while group B (n = 7), the higher group (microvesicular ≥ 10%). The mean total fatty change in the liver was 58.4% ± 47.2% and 67.6% ± 39.1% in groups A and B, respectively. A highly significant linear correlation between (1)H-MRS and total fatty change was observed in group A (r = .986, P < .001) while there was a relatively poor correlation in group B (r = .764, P = .05). The power to predict fatty change in the liver in groups A and B was significantly different (P = .004).
The degree of hepatic steatosis with a small amount of microvesicular steatosis (<10%) can be precisely predicted using 3-T (1)H-MRS.
肝脂肪变性（HS）在普通人群中较为常见，在供体器官中也存在，这可能会影响其移植存活率以及供体的恢复情况。我们的目标是开发一种在实验和临床方面都可靠的非侵入性方法，利用3-T氢磁共振波谱（MRS）对大泡性和小泡性肝脂肪变性进行量化。
通过给予缺乏甲硫氨酸胆碱和胆碱的饮食在大鼠中诱导大泡性和小泡性脂肪变性。使用MayoBC10线圈进行射频发射和信号接收。在3.0-T全身GE Signa系统上利用氢波谱对肝脏脂肪含量进行测量。脂肪（0.8 - 1.3 ppm）和水（4.7 ppm）曲线下面积的比值用于确定肝脏脂肪含量，并与组织病理学和生化脂肪变性程度进行比较。
根据小泡性肝脂肪变性的百分比将20只大鼠分为两组。A组（n = 13）为小泡性肝脂肪变性百分比较低的组（小泡性<10%），而B组（n = 7）为百分比更高的组（小泡性≥10%）。A组和B组肝脏总的脂肪变化均值分别为58.4%±47.2%和67.6%±39.1%。在A组中观察到氢磁共振波谱与总的脂肪变化之间存在高度显著的线性相关性（r = 0.986，P < 0.001），而在B组中相关性相对较差（r = 0.764，P = 0.05）。A组和B组预测肝脏脂肪变化的效能存在显著差异（P = 0.004）。
使用3-T氢磁共振波谱可以精确预测少量小泡性脂肪变性（<10%）的肝脂肪变性程度。
