Departamento de Promoción, Preservación y Desarrollo de la Salud, División Ciencias de la Salud, Laboratorio C de Memoria y Neuronutrición. Centro Universitario del Sur, Universidad de Guadalajara, Ciudad Guzmán, Jalisco, México.
Departamento de Neurobiología Celular y Molecular, Instituto de Neurobiología, Universidad Nacional Autónoma de México, Querétaro, México.
PLoS One. 2024 Jan 2;19(1):e0294062. doi: 10.1371/journal.pone.0294062. eCollection 2024.
Pregnancy is a challenging metabolic and physiological condition. The aim of this study was to include a second demanding situation as a low protein/high carbohydrate diet (LPHCD) to characterize the histological and functional responses of the maternal liver. It is unknown how the maternal liver responds during early and late pregnancy to LPHCD intake. We explored early pregnancy (3 and 8 gestational age, G) and late pregnancy (15 and 20 G). The results indicated that pregnant rats under control diet showed an evident presence of ballooned hepatocytes, lipid vesicles and edema at late pregnancy (15G); in contrast, pregnant rats under LPHCD showed similar pattern of histological modification but at early pregnancy (3G). Unexpectedly, the serum biomarkers didn't display functional alterations in either group, despite of the evident histological changes no liver malfunction was detected. We conclude that pregnant rats fed with control diet and experimental LPHCD, are subjected to metabolic and physiological conditions that impact the histopathological condition of the maternal liver. Control diet promoted the histological modifications during late pregnancy whereas LPCHCD advanced the onset of these changes. Further experiments are needed to explore the biochemical mechanisms that underlie these histological modifications. Our results are also an example of the resilience associated with the pregnancy: since no functional hepatic alterations accompanied the histopathological changes, another conclusion is that no evident pathological condition was detected in this nutritional protocol.
妊娠是一种具有挑战性的代谢和生理状态。本研究的目的是纳入第二种苛刻的情况，即低蛋白/高碳水化合物饮食（LPHCD），以描述母体肝脏的组织学和功能反应。目前尚不清楚母体肝脏在妊娠早期和晚期对 LPHCD 摄入的反应如何。我们探讨了妊娠早期（3 天和 8 天妊娠期，G）和妊娠晚期（15 天和 20 天 G）。结果表明，在妊娠晚期（15G），接受对照饮食的妊娠大鼠表现出明显的气球样肝细胞、脂滴和水肿；相比之下，在妊娠早期（3G）接受 LPHCD 的妊娠大鼠表现出类似的组织学改变模式。出乎意料的是，尽管组织学变化明显，但两组血清生物标志物均未显示出功能改变，没有检测到肝功能障碍。我们得出结论，接受对照饮食和实验性 LPHCD 的妊娠大鼠受到代谢和生理条件的影响，这些条件会影响母体肝脏的组织病理学状况。对照饮食促进了妊娠晚期的组织学改变，而 LPHCD 则提前了这些变化的发生。需要进一步的实验来探讨导致这些组织学改变的生化机制。我们的结果也是与妊娠相关的弹性的一个例子：由于组织病理学变化没有伴随肝功能改变，因此可以得出另一个结论，即在这种营养方案中没有检测到明显的病理状况。
