Vargas Angelica, Zeisser-Labouèbe Magali, Lange Norbert, Gurny Robert, Delie Florence
Department of Pharmaceutics and Biopharmaceutics, School of Pharmaceutical Sciences, University of Geneva, University of Lausanne, 30, Quai Ernest Ansermet, CH-1211 Geneva 4, Switzerland.
Adv Drug Deliv Rev. 2007 Sep 30;59(11):1162-76. doi: 10.1016/j.addr.2007.04.019. Epub 2007 Aug 16.
Mammalian models are frequently used for preclinical evaluation of new drug delivery systems (DDS). However, valid mammalian models are expensive, time-consuming, and not easy to set up and evaluate. Furthermore, they are often linked to administrative burden with respect to ethical and legal aspects. The present review outlines the possibilities and limitations of using the hen's embryo, and specifically its chorioallantoic membrane (CAM), as an alternative to mammalian models for the evaluation of DDS. Features of the CAM, the anatomy of the embryo, and the blood were investigated to assess properties of the drug carriers such as toxicity and biocompatibility, as well as the activity, toxicity, biodistribution and pharmacokinetics of the drug. The simplicity, rapidity, and low cost of the different assays that can be performed with chick embryos strengthen the interest of routinely using this model in pharmaceutical technology research. It is concluded that there is a big potential for using chick embryos in screening procedures of formulation candidates, thus establishing an intermediate step between in vitro cellular tests and preclinical mammalian models.
哺乳动物模型常用于新药递送系统（DDS）的临床前评估。然而，有效的哺乳动物模型成本高昂、耗时且不易建立和评估。此外，在伦理和法律方面，它们往往伴随着管理负担。本综述概述了使用鸡胚，特别是其绒毛尿囊膜（CAM）作为评估DDS的哺乳动物模型替代品的可能性和局限性。研究了CAM的特征、胚胎解剖结构和血液，以评估药物载体的特性，如毒性和生物相容性，以及药物的活性、毒性、生物分布和药代动力学。可以用鸡胚进行的不同检测的简单性、快速性和低成本，增强了在药物技术研究中常规使用该模型的兴趣。得出的结论是，在制剂候选物的筛选程序中使用鸡胚具有很大潜力，从而在体外细胞试验和临床前哺乳动物模型之间建立一个中间步骤。
