Sidhu Sachdev S, Koide Shohei
Department of Protein Engineering, Genentech, Inc., 1 DNA Way, South San Francisco, CA 94080, USA.
Curr Opin Struct Biol. 2007 Aug;17(4):481-7. doi: 10.1016/j.sbi.2007.08.007. Epub 2007 Sep 17.
Phage display is the longest-standing platform among molecular display technologies. Recent developments have extended its utility to proteins that were previously recalcitrant to phage display. The technique has played a dominant role in forming the field of synthetic binding protein engineering, where novel interfaces have been generated from libraries built using antibody fragment frameworks and also alternative scaffolds. Combinatorial methods have also been developed for the rapid analysis of binding energetics across protein interfaces. The ability to rapidly select and analyze binding interfaces, and compatibility with high-throughput methods under diverse conditions, makes it likely that the combination of phage display and synthetic combinatorial libraries will prove to be the method of choice for synthetic binding protein engineering for broad applications.
噬菌体展示是分子展示技术中存在时间最长的平台。最近的进展已将其应用扩展到以前难以用噬菌体展示的蛋白质。该技术在合成结合蛋白工程领域发挥了主导作用，在该领域中，利用抗体片段框架构建的文库以及其他支架产生了新的界面。还开发了组合方法用于快速分析蛋白质界面的结合能。能够快速选择和分析结合界面，以及在不同条件下与高通量方法的兼容性，使得噬菌体展示和合成组合文库的结合很可能被证明是广泛应用的合成结合蛋白工程的首选方法。
