Daugherty Patrick S
Department of Chemical Engineering, Biomolecular Science and Engineering, and Institute for Collaborative Biotechnologies, University of California, Santa Barbara, CA 93106, USA.
Curr Opin Struct Biol. 2007 Aug;17(4):474-80. doi: 10.1016/j.sbi.2007.07.004. Epub 2007 Aug 28.
Recent improvements in bacterial surface display systems coupled with efficient selection and screening strategies are propelling bacterial display systems to the forefront of peptide and protein engineering. The ability to analyze and screen very large protein libraries using cell-sorting instrumentation coupled with the ease of manipulating bacteria provides new capabilities for the protein engineering toolbox.
细菌表面展示系统的最新改进，再加上高效的选择和筛选策略，正推动细菌展示系统走向肽和蛋白质工程的前沿。利用细胞分选仪器分析和筛选非常大的蛋白质文库的能力，再加上操纵细菌的简便性，为蛋白质工程工具箱提供了新的功能。
