Ochoa María C, Santos José L, Azcona Cristina, Moreno-Aliaga María J, Martínez-González Miguel A, Martínez J Alfredo, Marti Amelia
Department of Nutrition and Food Sciences, Physiology and Toxicology, C/Irunlarrea s/n, University of Navarra, 31080 Pamplona, Spain.
Mol Genet Metab. 2007 Dec;92(4):351-8. doi: 10.1016/j.ymgme.2007.07.011. Epub 2007 Sep 17.
A number of studies have yielded controversial results on the association between polymorphisms in UCP2 and UCP3 genes with obesity and its comorbidities. The discrepancy among studies might be partially explained by the lack of consideration of the effect of adjacent loci in the same haplotype and the exclusion of key lifestyle factors in the statistical analysis. In this study, we have assessed the association between three genetic variants of the UCP2-UCP3 gene cluster, the -866G/A (rs659366) and the 45bp insertion (in position 173247 of the AC019121) of the UCP2 gene, the -55C/T (rs1800849) polymorphism of the UCP3 gene and their estimated haplotypes with childhood obesity and insulin resistance. This research was designed as a case-control study and information about several environmental parameters such as leisure time physical activity and time spent watching television were included. The study sample consisted in 193 obese children and adolescents (cases) and 170 controls aged 6-18. We found that the individual polymorphisms were not associated with obesity, but the (-866G; rs659366)-(Del; 45bp)-(-55T; rs1800849) haplotype was significantly associated with obesity and its presence in the control group increased about nine times the insulin resistance risk. Thus, the (-866A; rs659366)-(Ins; 45bp)-(-55C; rs1800849) haplotype may protect against insulin resistance in the obese population group.
多项研究在UCP2和UCP3基因多态性与肥胖及其合并症之间的关联上得出了有争议的结果。研究之间的差异可能部分归因于在统计分析中未考虑同一单倍型中相邻基因座的影响以及排除了关键生活方式因素。在本研究中，我们评估了UCP2 - UCP3基因簇的三个基因变体、UCP2基因的 - 866G/A（rs659366）和45bp插入（位于AC019121的第173247位）、UCP3基因的 - 55C/T（rs1800849）多态性及其估计单倍型与儿童肥胖和胰岛素抵抗之间的关联。本研究设计为病例对照研究，并纳入了有关休闲时间体育活动和看电视时间等几个环境参数的信息。研究样本包括193名肥胖儿童和青少年（病例）以及170名6 - 18岁的对照。我们发现个体多态性与肥胖无关，但（-866G；rs659366）-（缺失；45bp）-（-55T；rs1800849）单倍型与肥胖显著相关，且其在对照组中的存在使胰岛素抵抗风险增加了约九倍。因此，（-866A；rs659366）-（插入；45bp）-（-55C；rs1800849）单倍型可能对肥胖人群的胰岛素抵抗具有保护作用。
