高效液相色谱法测定生物体液中的长春碱、4-O-去乙酰长春碱及潜在代谢产物4-O-去乙酰长春碱-3-羧酸

High-performance liquid chromatographic determination of vinblastine, 4-O-deacetylvinblastine and the potential metabolite 4-O-deacetylvinblastine-3-oic acid in biological fluids.

作者信息

van Tellingen O, Beijnen J H, Baurain R, ten Bokkel Huinink W W, van der Woude H R, Nooyen W J

机构信息

Department of Clinical Chemistry, Netherlands Cancer Institute, Amsterdam.

出版信息

J Chromatogr. 1991 Aug 16;553(1-2):47-53. doi: 10.1016/s0021-9673(01)88471-5.

DOI:10.1016/s0021-9673(01)88471-5

PMID:1787167

Abstract

Procedures for the determination of vinblastine (VBL), 4-O-deacetylvinblastine (DVBL) and 4-O-deacetylvinblastine-3-oic acid (DVBLA) in biological samples using high-performance liquid chromatography (HPLC) combined with selective sample clean-up are presented. VBL and DVBL were determined in plasma and urine using ion-exchange normal-phase HPLC with fluorescence detection. The limit of detection was 1 microgram/l for both compounds using a 500-microliter sample. Successful chromatographic analyses of DVBLA were achieved by using a glass column packed with 5-microns Hypersil ODS and acetonitrile-0.05 M phosphate buffer (pH 2.7) (23:77, v/v). Positive identification was supported by the use of diode-array detection. The limit of detection (at 270 nm) was 10 micrograms/l using 1-ml samples.

摘要

本文介绍了使用高效液相色谱（HPLC）结合选择性样品净化法测定生物样品中长春碱（VBL）、4-O-去乙酰长春碱（DVBL）和4-O-去乙酰长春碱-3-酸（DVBLA）的方法。采用离子交换正相HPLC结合荧光检测法测定血浆和尿液中的VBL和DVBL。使用500微升样品时，两种化合物的检测限均为1微克/升。通过使用填充有5微米Hypersil ODS的玻璃柱和乙腈-0.05 M磷酸盐缓冲液（pH 2.7）（23:77，v/v），成功实现了DVBLA的色谱分析。使用二极管阵列检测支持阳性鉴定。使用1毫升样品时，（在270纳米处）检测限为10微克/升。