Sóczó G, Kardos G, McNicholas P M, Balogh E, Gergely L, Varga I, Kelentey B, Majoros L
Department of Medical Microbiology, Medical and Health Science Center, University of Debrecen, Hungary.
J Antimicrob Chemother. 2007 Nov;60(5):1004-9. doi: 10.1093/jac/dkm350. Epub 2007 Sep 17.
We evaluated the in vitro activity of posaconazole against nine Candida species using minimum fungicidal concentration (MFC) measurements and time-kill methods.
MFCs of posaconazole were determined for 209 clinical isolates (32 Candida albicans, 30 Candida glabrata, 21 Candida tropicalis, 29 Candida krusei, 28 Candida parapsilosis sensu stricto, 50 Candida inconspicua, 13 Candida kefyr, 3 Candida lusitaniae and 3 Candida guilliermondii) and 7 ATCC Candida strains. The following strains were tested in time-kill studies: 3 strains each of C. glabrata, C. kefyr, C. guilliermondii and C. lusitaniae; 2 C. tropicalis; 4 C. albicans; 4 C. inconspicua; 9 C. krusei; 12 C. parapsilosis; and 7 ATCC strains.
Posaconazole was fungicidal in both MFC and time-kill experiments (at 2 mg/L within 48 h in time-kill assays) against each C. krusei, C. inconspicua and C. lusitaniae strain and was fungistatic against each C. albicans, C. glabrata, C. tropicalis and C. guilliermondii strain. For the C. parapsilosis strains, posaconazole MFCs were <or=2 mg/L for 20 of the 28 isolates tested; however, in the time-kill tests, even at drug concentrations of 32-64 x MIC, posaconazole was fungistatic against all C. parapsilosis strains tested.
MFC values were good predictors of the fungistatic or fungicidal activity of posaconazole, as determined by time-kill curves, for all tested species except C. parapsilosis. Our results highlight the fungicidal action of posaconazole against a number of clinically relevant Candida species.
我们使用最低杀菌浓度（MFC）测定法和时间-杀菌方法评估了泊沙康唑对9种念珠菌的体外活性。
测定了泊沙康唑对209株临床分离株（32株白色念珠菌、30株光滑念珠菌、21株热带念珠菌、29株克柔念珠菌、28株近平滑念珠菌、50株无名念珠菌、13株解脂念珠菌、3株葡萄牙念珠菌和3株季也蒙念珠菌）以及7株美国典型培养物保藏中心（ATCC）念珠菌菌株的MFC。在时间-杀菌研究中测试了以下菌株：光滑念珠菌、解脂念珠菌、季也蒙念珠菌和葡萄牙念珠菌各3株；热带念珠菌2株；白色念珠菌4株；无名念珠菌4株；克柔念珠菌9株；近平滑念珠菌12株；以及7株ATCC菌株。
在MFC和时间-杀菌实验中（在时间-杀菌试验中2 mg/L时48小时内），泊沙康唑对各株克柔念珠菌、无名念珠菌和葡萄牙念珠菌具有杀菌作用，而对各株白色念珠菌、光滑念珠菌、热带念珠菌和季也蒙念珠菌具有抑菌作用。对于近平滑念珠菌菌株，在测试的28株分离株中，20株的泊沙康唑MFC≤2 mg/L；然而，在时间-杀菌试验中，即使药物浓度为32 - 64倍MIC，泊沙康唑对所有测试的近平滑念珠菌菌株仍具有抑菌作用。
对于除近平滑念珠菌外的所有测试菌种，MFC值是由时间-杀菌曲线确定的泊沙康唑抑菌或杀菌活性的良好预测指标。我们的结果突出了泊沙康唑对多种临床相关念珠菌菌种的杀菌作用。
