Baharuddin Nur Saeida, Abdullah Hasmah, Abdul Wahab Wan Nor Amilah Wan
Biomedicine Programme, School of Health Sciences, Universiti Sains Malaysia, Kubang Kerian 16150, Kelantan.
J Pharm Bioallied Sci. 2015 Jan-Mar;7(1):15-20. doi: 10.4103/0975-7406.148742.
Galls of Quercus infectoria have been traditionally used to treat common ailments, including yeast infections caused by Candida species.
This study aimed to evaluate the in vitro anti-Candida activity of Q. infectoria gall extracts against selected Candida species.
Methanol and aqueous extracts of Q. infectoria galls were tested for anti-Candida activity against Candida albicans, Candida krusei, Candida glabrata, Candida parapsilosis and Candida tropicalis. The minimum inhibitory concentrations were determined using the two-fold serial dilution technique of concentrations ranging from 16 mg/ml to 0.03 mg/ml. After 24 h, the minimum fungicidal concentrations were determined by subculturing the wells, which showed no turbidity on the agar plate. Potential phytochemical group in the crude extracts was screened by phytochemical qualitative tests and subsequently subjected to the gas chromatography-mass spectrometry analysis.
Both methanol and aqueous extracts displayed substantial anti-Candida activity and pyrogallol was the major component of both crude extracts.
Data from current study suggested that Q. infectoria gall extracts are a potential source to be developed as anti-candidiasis.
没食子传统上用于治疗常见疾病，包括念珠菌属引起的酵母菌感染。
本研究旨在评估没食子提取物对所选念珠菌属的体外抗念珠菌活性。
测试没食子的甲醇提取物和水提取物对白色念珠菌、克柔念珠菌、光滑念珠菌、近平滑念珠菌和热带念珠菌的抗念珠菌活性。使用浓度范围为16mg/ml至0.03mg/ml的两倍系列稀释技术确定最低抑菌浓度。24小时后，通过将琼脂平板上无浑浊的孔传代培养来确定最低杀菌浓度。通过植物化学定性试验筛选粗提取物中的潜在植物化学基团，随后进行气相色谱-质谱分析。
甲醇提取物和水提取物均表现出显著的抗念珠菌活性，焦性没食子酚是两种粗提取物的主要成分。
当前研究数据表明，没食子提取物是一种有潜力开发为抗念珠菌病药物的来源。
