Hanahan Douglas, Wagner Erwin F, Palmiter Richard D
Department of Biochemistry and Biophysics, Diabetes Center, and Comprehensive Cancer Center, University of California at San Francisco, San Francisco, CA 94143, USA.
Genes Dev. 2007 Sep 15;21(18):2258-70. doi: 10.1101/gad.1583307.
This perspective describes the concurrent development in the 1980s of the first transgenic mice genetically engineered to express dominant oncogenes, involving independent researchers who were largely unaware of each other's strategies and progress. We relate the experimental designs, the pitfalls and challenges encountered, and the eventual success in developing distinctive mouse models of cancer, wherein tumors arose heritably in various organs. These early oncomice have produced a wealth of new knowledge, become topics of intellectual property, and spawned a vibrant field of cancer research that is revealing mechanisms of tumorigenesis and suggesting new therapeutic strategies for treating the human disease.
这篇综述描述了20世纪80年代首批经基因工程改造以表达显性癌基因的转基因小鼠的同步研发情况，涉及一些彼此基本互不了解对方策略和进展的独立研究人员。我们阐述了实验设计、所遇到的陷阱与挑战，以及最终成功开发出独特的癌症小鼠模型的过程，在这些模型中，肿瘤可在各种器官中遗传性地出现。这些早期的致癌小鼠产生了大量新知识，成为知识产权的主题，并催生了一个充满活力的癌症研究领域，该领域正在揭示肿瘤发生机制，并为治疗人类疾病提出新的治疗策略。
