Comar Manola, Rizzardi Clara, de Zotti Renata, Melato Mauro, Bovenzi Massimo, Butel Janet S, Campello Cesare
Department of Public Medicine Sciences, University of Trieste, Trieste, Italy.
Cancer Res. 2007 Sep 15;67(18):8456-9. doi: 10.1158/0008-5472.CAN-07-2232.
To assess the presence of SV40 in malignant mesothelioma tissue, 19 formalin-fixed paraffin-embedded pleural cancer samples of patients from a hyperendemic area of northeastern Italy were analyzed retrospectively. A total of 48 other tissues from the malignant mesothelioma subjects were investigated. The SV40 load was determined by real-time quantitative PCR. Exposure to asbestos was evaluated through a careful review of the occupational history of patients, supplemented by histology and isolation of asbestos bodies. Three of 19 (15.8%) malignant mesothelioma tissues harbored SV40 genomic signals. Two patients with SV40-positive malignant mesothelioma had viral sequences in another tissue. Overall, 3 of 18 (16.7%) normal liver tissues tested positive for SV40, as did 1 of 8 (12.5%) kidney tissues. SV40 viral loads were higher in malignant mesothelioma than in normal cells (P = 0.045). This survey shows that SV40 sustains infections in multiple tissues in malignant mesothelioma patients from a geographic area affected with asbestos-related mesothelioma.
为评估恶性间皮瘤组织中是否存在SV40，我们对来自意大利东北部高流行区的19例患者的福尔马林固定石蜡包埋胸膜癌样本进行了回顾性分析。对恶性间皮瘤患者的另外48份组织进行了研究。通过实时定量PCR测定SV40载量。通过仔细回顾患者职业史，并辅以组织学检查和石棉小体分离来评估石棉暴露情况。19份恶性间皮瘤组织中有3份（15.8%）含有SV40基因组信号。两名SV40阳性恶性间皮瘤患者的另一组织中也有病毒序列。总体而言，18份正常肝组织中有3份（16.7%）SV40检测呈阳性，8份肾组织中有1份（12.5%）呈阳性。恶性间皮瘤中的SV40病毒载量高于正常细胞（P = 0.045）。这项调查表明，在受石棉相关间皮瘤影响的地理区域，恶性间皮瘤患者的多种组织中存在SV40感染。
