A类碳青霉烯水解β-内酰胺酶SFC-1的生化特性
Biochemical Characterization of SFC-1, a class A carbapenem-hydrolyzing beta-lactamase.
作者信息
Fonseca Fátima, Sarmento Ana Cristina, Henriques Isabel, Samyn Bart, van Beeumen Jozef, Domingues Pedro, Domingues Maria Rosário, Saavedra Maria José, Correia António
机构信息
Centre for Environmental and Marine Studies, Department of Biology, University of Aveiro, 3810-193 Aveiro, Portugal.
出版信息
Antimicrob Agents Chemother. 2007 Dec;51(12):4512-4. doi: 10.1128/AAC.00491-07. Epub 2007 Sep 17.
Abstract
The carbapenem-hydrolyzing beta-lactamase SFC-1 from Serratia fonticola UTAD54 was overexpressed in Escherichia coli, purified, and characterized. The enzyme exhibited an apparent molecular mass of 30.5 kDa, determined by sodium dodecyl sulfate-polyacrylamide gel electrophoresis. SFC-1 hydrolyzes penicillins, cephalosporins, aztreonam, and carbapenems and is inhibited by clavulanic acid, sulbactam, and tazobactam.
摘要
来自丰源沙雷氏菌UTAD54的碳青霉烯水解β-内酰胺酶SFC-1在大肠杆菌中过表达、纯化并进行了表征。通过十二烷基硫酸钠-聚丙烯酰胺凝胶电泳测定，该酶的表观分子量为30.5 kDa。SFC-1可水解青霉素、头孢菌素、氨曲南和碳青霉烯类，并且受克拉维酸、舒巴坦和他唑巴坦抑制。
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