Lee Soo-Ung, Chun Ha-Chung, Huh Sun
Department of Environmental and Tropical Medicine, School of Medicine, KonKuk University, Chungju, Republic of Korea.
Korean J Parasitol. 2007 Sep;45(3):181-9. doi: 10.3347/kjp.2007.45.3.181.
The phylogenic relationships existing among 14 parasitic Platyhelminthes in the Republic of Korea were investigated via the use of the partial 28S ribosomal DNA (rDNA) D1 region and the partial mitochondrial cytochrome c oxidase subunit 1 (mCOI) DNA sequences. The nucleotide sequences were analyzed by length, G + C %, nucleotide differences and gaps in order to determine the analyzed phylogenic relationships. The phylogenic patterns of the 28S rDNA D1 and mCOI regions were closely related within the same class and order as analyzed by the PAUP 4.0 program, with the exception of a few species. These findings indicate that the 28S rDNA gene sequence is more highly conserved than are the mCOI gene sequences. The 28S rDNA gene may prove useful in studies of the systematics and population genetic structures of parasitic Platyhelminthes.
通过使用部分28S核糖体DNA（rDNA）D1区域和部分线粒体细胞色素c氧化酶亚基1（mCOI）DNA序列，对韩国14种寄生扁形虫之间存在的系统发育关系进行了研究。为了确定所分析的系统发育关系，对核苷酸序列进行了长度、G + C%、核苷酸差异和缺口分析。通过PAUP 4.0程序分析，28S rDNA D1和mCOI区域的系统发育模式在同一纲和目内密切相关，但有少数物种除外。这些发现表明，28S rDNA基因序列比mCOI基因序列更具保守性。28S rDNA基因可能在寄生扁形虫的系统学和种群遗传结构研究中证明是有用的。
