Lerescu L, Tucureanu C, Caraş Iuliana, Sălăgeanu Aurora
Infection and Immunity Laboratory, "Cantacuzino" National Institute of Research and Development for Microbiology and Immunology, Bucharest, Romania.
Roum Arch Microbiol Immunol. 2006 Jan-Jun;65(1-2):53-8.
The bacterial product CANTASTIM (CS) is a purified extract of Pseudomonas aeruginosa that induces non-specific protection against bacterial infection, enhances macrophage effector functions and modulates production of cytokines. Most likely, it interacts with components of the innate immune response. Cytokine production can be used to assess the bioactivity of this product but these biomolecules operate in vivo in a complex regulatory network with reciprocal influences so there is a need for profiling an array of cytokines rather than an individual analysis. Current technology development of multiplex immunoassay for simultaneous measurement of multiple analytes in a single assay has greatly improved the throughput and cost effectiveness of cytokine profiling and proved to be an effective approach to evaluate the immunomodulatory activity of the bacterial product CS.
细菌产物CANTASTIM（CS）是铜绿假单胞菌的纯化提取物，可诱导对细菌感染的非特异性保护，增强巨噬细胞效应功能并调节细胞因子的产生。它很可能与先天免疫反应的成分相互作用。细胞因子的产生可用于评估该产物的生物活性，但这些生物分子在体内以复杂的调节网络运作，相互影响，因此需要对一系列细胞因子进行分析，而不是单独分析。用于在单一检测中同时测量多种分析物的多重免疫检测技术的当前发展，极大地提高了细胞因子分析的通量和成本效益，并被证明是评估细菌产物CS免疫调节活性的有效方法。
