Melinceanu L, Sarafoleanu C, Lerescu L, Tucureanu C, Caraş I, Sălăgeanu A
"Sfânta Maria" ENT-CCF Clinic, Bucharest, Romania.
J Med Life. 2009 Apr-Jun;2(2):211-8.
Immunity plays an important role in the prognosis and the natural development of cancer. Previous studies have shown that the presence of tumor in the body could modify the immune response leading to immunosuppression. The aim of this study was to evaluate the immunological changes of patients with larynx squamous cell carcinoma undergoing potentially curative surgery. We assessed the serum levels of cytokines (IL-1, IL-2, IL-4, IL-6, IL-8, IL-10, IFN-gamma, TNF-alpha, GM-CSF), chemokines (MCP-1 and MIP-1alpha) and growth factors (VEGF and bFGF) in laryngeal cancer patients before, during and after surgery. We have used a novel multianalyte XMap profiling technology that allows simultaneous measurement of multiple parameters in small volumes of samples. To investigate the changes in immune mediators' profile induced by tumor resection, we assessed the culture supernatants of the peripheral blood mononuclear cells (PBMC) derived from the patients, before and after surgery. The results suggested a predominance of a Th2 type of immune response associated with the presence of the tumor (especially in the case of heavy smokers who smoke more than 40 pack-years). However, shifts towards a Th1 type of immune response as well as an improvement of monocyte functions were noticed after surgery.
免疫在癌症的预后和自然发展过程中起着重要作用。先前的研究表明，体内肿瘤的存在会改变免疫反应，导致免疫抑制。本研究的目的是评估接受潜在根治性手术的喉鳞状细胞癌患者的免疫变化。我们评估了喉癌患者手术前、手术期间和手术后血清中细胞因子（IL-1、IL-2、IL-4、IL-6、IL-8、IL-10、IFN-γ、TNF-α、GM-CSF）、趋化因子（MCP-1和MIP-1α）和生长因子（VEGF和bFGF）的水平。我们使用了一种新型的多分析物XMap分析技术，该技术能够在少量样本中同时测量多个参数。为了研究肿瘤切除引起的免疫介质谱的变化，我们评估了患者手术前后外周血单个核细胞（PBMC）的培养上清液。结果表明，与肿瘤存在相关的是Th2型免疫反应占主导（尤其是对于吸烟超过40包年的重度吸烟者）。然而，手术后观察到向Th1型免疫反应的转变以及单核细胞功能的改善。
