Marino A, Crupi R, Rizzo G, Morabito R, Musci G, La Spada G
University of Messina, Department of General Physiology and Pharmacology, Salita Sperone 31, 98166 Messina, Italy.
Cell Mol Biol (Noisy-le-grand). 2007 Sep 17;53 Suppl:OL994-1002.
We have firstly investigated the toxicological activity by hemolytic assay of crude extract obtained by sonication of holotrichous isorhiza isolated nematocysts of the Scyphozoan Pelagia noctiluca, collected in the Strait of Messina. The hemolytic activity was both time- and dose-dependent on fish, rabbit, chicken and human red blood cells. At lowest doses rabbit and chicken erythrocytes were the most sensitive, whereas those of eel were the most resistant to the crude extract. Different storage conditions, such as -20 degrees C, -80 degrees C for up to 6 months and lyophilization, did not affect the stability of crude venom. Moreover, neither treatment at 4 degrees C, 20 degrees C and 37 degrees C for different time periods ranging between 30 min and 24 h, nor harsh thermal treatment at 80 degrees C and 100 degrees C affected the hemolytic power. The crude venom resulted even stable towards proteolysis and alkaline pH values.
我们首先通过溶血试验研究了从墨西拿海峡采集的夜光游水母钵水母全毛等辐骨虫分离的刺丝囊经超声处理得到的粗提物的毒理学活性。溶血活性对鱼、兔、鸡和人红细胞均呈时间和剂量依赖性。在最低剂量下，兔和鸡的红细胞最敏感，而鳗鱼的红细胞对粗提物最具抗性。不同的储存条件，如-20℃、-80℃长达6个月以及冻干，均不影响粗毒液的稳定性。此外，在4℃、20℃和37℃下分别处理30分钟至24小时的不同时间段，以及在80℃和100℃下进行的严苛热处理，均不影响溶血能力。粗毒液对蛋白水解和碱性pH值也具有稳定性。
