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毒液的溶血、抗癌及抗贾第虫活性。

Hemolytic, anticancer and antigiardial activity of venom.

作者信息

Lazcano-Pérez Fernando, Zavala-Moreno Ariana, Rufino-González Yadira, Ponce-Macotela Martha, García-Arredondo Alejandro, Cuevas-Cruz Miguel, Gómez-Manzo Saúl, Marcial-Quino Jaime, Arreguín-Lozano Barbarín, Arreguín-Espinosa Roberto

机构信息

1Departamento de Química de Biomacromoléculas, Instituto de Química, Universidad Nacional Autónoma de México, Av. Universidad 3000, Ciudad Universitaria, C.P. 04510. Apdo, Postal 70250 Mexico City, Mexico.

2Laboratorio de Parasitología Experimental, Instituto Nacional de Pediatría, Insurgentes Sur 3700-C, 04530 Mexico City, Mexico.

出版信息

J Venom Anim Toxins Incl Trop Dis. 2018 Apr 17;24:12. doi: 10.1186/s40409-018-0149-8. eCollection 2018.

DOI:10.1186/s40409-018-0149-8
PMID:29692802
原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC5905176/
Abstract

BACKGROUND

Cnidarian venoms and extracts have shown a broad variety of biological activities including cytotoxic, antibacterial and antitumoral effects. Most of these studied extracts were obtained from sea anemones or jellyfish. The present study aimed to determine the toxic activity and assess the antitumor and antiparasitic potential of venom by evaluating its in vitro toxicity on several models including human tumor cell lines and against the parasite .

METHODS

The presence of cytolysins and vasoconstrictor activity of venom were determined by hemolysis, PLA and isolated rat aortic ring assays, respectively. The cytotoxic effect was tested on HCT-15 (human colorectal adenocarcinoma), MCF-7 (human mammary adenocarcinoma), K562 (human chronic myelogenous leukemia), U251 (human glyoblastoma), PC-3 (human prostatic adenocarcinoma) and SKLU-1 (human lung adenocarcinoma). An in vivo toxicity assay was performed with crickets and the antiparasitic assay was performed against at 24 h of incubation.

RESULTS

venom produced hemolytic and PLA activity and showed specific cytotoxicity against U251 and SKLU-1 cell lines, with approximately 50% growing inhibition. The venom was toxic to insects and showed activity against in a dose-dependent manner by possibly altering its membrane osmotic equilibrium.

CONCLUSION

These results suggest that venom contains compounds with potential therapeutic value against microorganisms and cancer.

摘要

背景

刺胞动物毒液和提取物已显示出多种生物活性，包括细胞毒性、抗菌和抗肿瘤作用。这些研究的提取物大多来自海葵或水母。本研究旨在通过评估其对包括人类肿瘤细胞系在内的多种模型以及对寄生虫的体外毒性，来确定毒液的毒性活性并评估其抗肿瘤和抗寄生虫潜力。

方法

分别通过溶血、血小板聚集（PLA）和离体大鼠主动脉环试验来确定毒液中溶细胞素的存在和血管收缩活性。对HCT-15（人结肠腺癌）、MCF-7（人乳腺腺癌）、K562（人慢性粒细胞白血病）、U251（人胶质母细胞瘤）、PC-3（人前列腺腺癌）和SKLU-1（人肺腺癌）进行细胞毒性测试。用蟋蟀进行体内毒性试验，并在孵育24小时后对[寄生虫名称缺失]进行抗寄生虫试验。

结果

毒液产生溶血和PLA活性，并对U251和SKLU-1细胞系表现出特异性细胞毒性，生长抑制率约为50%。该毒液对昆虫有毒，并可能通过改变其膜渗透平衡对[寄生虫名称缺失]表现出剂量依赖性活性。

结论

这些结果表明，[毒液名称缺失]毒液含有对微生物和癌症具有潜在治疗价值的化合物。

https://cdn.ncbi.nlm.nih.gov/pmc/blobs/f0e7/5905176/b6f2ba3bba1c/40409_2018_149_Fig5_HTML.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/f0e7/5905176/80f07d74a966/40409_2018_149_Fig1_HTML.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/f0e7/5905176/25e001b70404/40409_2018_149_Fig2_HTML.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/f0e7/5905176/4d84d28b3803/40409_2018_149_Fig3_HTML.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/f0e7/5905176/50ab1004ce1e/40409_2018_149_Fig4_HTML.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/f0e7/5905176/b6f2ba3bba1c/40409_2018_149_Fig5_HTML.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/f0e7/5905176/80f07d74a966/40409_2018_149_Fig1_HTML.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/f0e7/5905176/25e001b70404/40409_2018_149_Fig2_HTML.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/f0e7/5905176/4d84d28b3803/40409_2018_149_Fig3_HTML.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/f0e7/5905176/50ab1004ce1e/40409_2018_149_Fig4_HTML.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/f0e7/5905176/b6f2ba3bba1c/40409_2018_149_Fig5_HTML.jpg

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